HA tag antibody

Catalogue No.:FNab03742Reactivity:recombinant protein, Human
Host:RabbitTested Application:ELISA, WB, IP, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
HA tag antibody
Catalogue No.
FNab03742
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
HA tag
Application
Tested Applications
ELISA, WB, IP, IF
Recommended dilution
WB: 1:1000-1:8000; IP: 1:1000-1:4000; IF: 1:10-1:100
Validated Images
HA-tagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03742(HA tag Antibody) at dilution of 1:4000
IP Result of anti-HA tag (IP: FNab03742, 3ug; Detection: FNab03742 1:2000) with Transfected HEK-293 cells lysate 500ug
Background
The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.