Products
GPR177 antibody
- Product Name
- GPR177 antibody
- Catalogue No.
- FNab03601
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- G protein-coupled receptor 177
- Alternative Names
- C1orf139 antibody, EVI antibody, G protein coupled receptor 177 antibody, GPR177 antibody, Protein wntless homolog antibody, Sprinter antibody, WLS antibody, Wntless antibody
- UniProt ID
- Q5T9L3
- Observed MW
- 46-62 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200
Validated Images
mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab03601(GPR177 antibody) at dilution of 1:500
IP Result of anti-GPR177 (IP: FNab03601, 3ug; Detection: FNab03601 1:500) with mouse liver tissue lysate 10000ug.
Immunohistochemistry of paraffin-embedded human ovary using FNab03601(GPR177 antibody) at dilution of 1:100
- Background
- GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).
