Products
FHIT antibody
- Product Name
- FHIT antibody
- Catalogue No.
- FNab03108
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 8G4
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- fragile histidine triad gene
- Alternative Names
- AP3A hydrolase antibody, AP3Aase antibody, Bis(5 adenosyl) triphosphatase antibody, Dinucleosidetriphosphatase antibody, FHIT antibody, FRA3B antibody, fragile histidine triad gene antibody
- UniProt ID
- P49789
- Observed MW
- 17 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC
- Recommended dilution
- WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
验证图片
K-562 cells were subjected to SDS PAGE followed by western blot with FNab03108(FHIT antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human breast cancer slide using FNab03108(FHIT Antibody) at dilution of 1:50
- Background
- Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate(Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate(Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.