Products
DNMT3A antibody
- Product Name
- DNMT3A antibody
- Catalogue No.
- FNab02485
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- DNA(cytosine-5-)-methyltransferase 3 alpha
- UniProt ID
- Q9Y6K1
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab02485(DNMT3A antibody) at dilution of 1:1500
IP Result of anti-DNMT3A (IP:FNab02485, 4ug; Detection:FNab02485 1:1000) with HeLa cells lysate 2000ug.
- Background
- Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3(H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
