Products
DAB2 antibody
- Product Name
- DAB2 antibody
- Catalogue No.
- FNab02228
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- disabled homolog 2, mitogen-responsive phosphoprotein(Drosophila)
- Alternative Names
- DAB2 antibody, Disabled homolog 2 antibody, DOC 2 antibody, DOC2 antibody, FLJ26626 antibody
- UniProt ID
- P98082
- Observed MW
- 96 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, FC, IP
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:500-1:2000; IF: 1:20-1:200; IHC: 1:50-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02228(DAB2 antibody) at dilution of 1:1000
IP Result of anti-DAB2 (IP:FNab02228, 4ug; Detection:FNab02228 1:2000) with HeLa cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab02228(DAB2 Antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells, using DAB2 antibody FNab02228 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Background
- Adapter protein that functions as clathrin-associated sorting protein(CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate(PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Involved in CFTR trafficking to the late endosome. Involved in several receptor-mediated signaling pathways. Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2. Mediates TFG-beta-stimulated JNK activation. May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1(PP1). Sequesters LRP6 towards clathrin-mediated endocytosis, leading to inhibition of Wnt/beta-catenin signaling. May activate non-canonical Wnt signaling. In cell surface growth factor/Ras signaling pathways proposed to inhibit ERK activation by interrupting the binding of GRB2 to SOS1 and to inhibit SRC by preventing its activating phosphorylation at 'Tyr-419'. Proposed to be involved in modulation of androgen receptor(AR) signaling mediated by SRC activation; seems to compete with AR for interaction with SRC. Plays a role in the CSF-1 signal transduction pathway. Plays a role in cellular differentiation. Involved in cell positioning and formation of visceral endoderm(VE) during embryogenesis and proposed to be required in the VE to respond to Nodal signaling coming from the epiblast. Required for the epithelial to mesenchymal transition, a process necessary for proper embryonic development. May be involved in myeloid cell differentiation and can induce macrophage adhesion and spreading. May act as a tumor suppressor.
