Products
BrdU antibody
- Product Name
- BrdU antibody
- Catalogue No.
- FNab00952
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 9E8
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- compound
- Alternative Names
- 5-bromo-2-deoxyuridine antibody, BrDu antibody, BrdUrd antibody, Bromodeoxyuridine antibody, BUdR antibody
Application
- Tested Applications
- ELISA, IF, IHC
- Recommended dilution
- IHC: 1:200-1:1000; IF: 1:50-1:500
Validated Images
Immunohistochemistry of paraffin-embedded mouse spleen tissue slide(with BrdU feeding) using FNab00952(BrDu Antibody) at dilution of 1:400. Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using FNab00952 ( BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Background
- Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
