Products
BAAT antibody
- Product Name
- BAAT antibody
- Catalogue No.
- FNab00780
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- bile acid Coenzyme A: amino acid N-acyltransferase(glycine N-choloyltransferase)
- UniProt ID
- Q14032
- Observed MW
- 50kd
Application
- Tested Applications
- ELISA, WB, IF, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
human liver tissue were subjected to SDS PAGE followed by western blot with FNab00780(BAAT antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using FNab00780(BAAT Antibody) at dilution of 1:100
- Background
- Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids(choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes(primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum(secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation(secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long-and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A(CoASH), and conjugates glycine to these acyl-CoAs.
