mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab00749(ENPP2 antibody) at dilution of 1:500
IP Result of anti-Autotaxin (IP:FNab00749, 5ug; Detection:FNab00749 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab00749(ENPP2 Antibody) at dilution of 1:50
Background
Hydrolyzes lysophospholipids to produce lysophosphatidic acid(LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.