Products
Alpha-1-Antitrypsin antibody
Category:
Research Area:
Synonyms: | A1A antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody, PI antibody, PI1 antibody, PRO2209 antibody, PRO2275 antibody, Serpin A1 antibody, SERPINA1 antibody, SPAAT antibody, α1-antitrypsin antibody | ||
Catalogue No.: | FNab00338 | Reactivity: | Human, Rat |
Host: | Mouse | Tested Application: | ELISA, WB, IHC, IF |
Clonality: | monoclonal | Isotype: | IgG1 |
- SPECIFICATIONS
- Product Name
- Alpha-1-Antitrypsin antibody
- Catalogue No.
- FNab00338
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 5B1
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 1
- Alternative Names
- A1A antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody, PI antibody, PI1 antibody, PRO2209 antibody, PRO2275 antibody, Serpin A1 antibody, SERPINA1 antibody, SPAAT antibody, α1-antitrypsin antibody
- UniProt ID
- P01009
- Observed MW
- 50 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:500-1:2000; IF: 1:20-1:200; IHC: 1:500-1:1000
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00338(Alpha-1-Antitrypsin antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver using FNab00338(Alpha-1-Antitrypsin antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells using FNab00338( Alpha-1-Antitrypsin Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Background
- SERPINA1 is the gene for a protein called alpha-1-antitrypsin(AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.