Products
alpha Tubulin antibody
- Product Name
- alpha Tubulin antibody
- Catalogue No.
- FNab00335
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2b
- Clone ID
- 4A10
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tubulin, alpha 1b
- Alternative Names
- Alpha Tubulin antibody, Alpha tubulin ubiquitous antibody, alpha tubulin antibody, a tubulin antibody, TUBA1B antibody, tubulin antibody, tubulin antibody, alpha 1b antibody
- UniProt ID
- P68363
- Observed MW
- 50-55 kDa
Application
- Tested Applications
- ELISA, IHC, IF, WB, FC, IP
- Recommended dilution
- WB: 1:2000-1:10000; IP: 1:500-1:5000; IF: 1:50-1:500; IHC: 1:50-1:500
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab00335(alpha Tubulin Antibody) at dilution of 1:10000
IP Result of anti-alpha Tubulin (IP: FNab00335, 4ug; Detection: FNab00335 1:1000) with HeLa cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab00335(alpha Tubulin Antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using FNab00335 ( alpha Tubulin Antibody) at dilution of 1:100 and CY3-Goat anti-Rabbit IgG
- Background
- There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
