Products
AKAP8L antibody
- Product Name
- AKAP8L antibody
- Catalogue No.
- FNab00258
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- A kinase(PRKA) anchor protein 8-like
- Alternative Names
- A kinase anchor protein 8 like antibody, AKAP8 like protein antibody, AKAP8L antibody, DKFZp434L0650 antibody, HA95 antibody, HAP95 antibody, Helicase A binding protein 95 antibody, Homologous to AKAP95 protein antibody, NAKAP antibody, NAKAP95 antibody, Neighbor of AKAP95 antibody
- UniProt ID
- Q9ULX6
- Observed MW
- 72 kDa
Application
- Tested Applications
- ELISA, WB, IF
- Recommended dilution
- WB: 1:200-1:2000; IF: 1:20-200
Validated Images
PC-3 cells were subjected to SDS PAGE followed by western blot with FNab00258(AKAP8L Antibody) at dilution of 1:300
- Background
- Could play a role in constitutive transport element(CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export(PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA(PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis(PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta(PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8(PubMed:16980585). May be involved in regulation of pre-mRNA splicing(PubMed:17594903). (Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters(PubMed:11884601). Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses(PubMed:11402034). In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity(PubMed:25034436).
