COVID-19 Spike protein(S1) ELISA Kit

Category:
Catalogue No.: 
EU2605
Alias: 
COVID-19 S1 ELISA Kit
Species: 
Coronavirus(COVID-19)
Range: 
1.563-100pg/ml
Sensitivity: 
0.938pg/ml
Manual MSDS
  • SPECIFICATIONS
Product Name
COVID-19 Spike protein(S1) ELISA Kit
Alias
COVID-19 S1 ELISA Kit
Catalogue No.
EU2605
Size
48T/96T
Species
Coronavirus(COVID-19)
Detection Method
Sandwich ELISA, Double Antibody
Detection Wavelength
OD450
Reaction Duration
4 hours
Range
1.563-100pg/ml
Sensitivity
0.938pg/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with COVID-19 S1 , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml) OD-1 OD-2 Average
0 0.109 0.114 0.111
1.562 0.162 0.17 0.165
3.125 0.198 0.208 0.202
6.25 0.31 0.325 0.316
12.5 0.504 0.529 0.514
25 0.865 0.909 0.883
50 1.395 1.466 1.423
100 2.13 2.238 2.173
EU2605 Standard Curve Image
Recovery

Add a certain amount of COVID-19 S1 into the sample. Calculate the recovery by comparing the measured value with the expected amount of COVID-19 S1 in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 92-104 98
EDTA plasma(n=10) 85-101 95
Heparin plasma(n=10) 87-105 94
Linearity

Dilute the sample with a certain amount of COVID-19 S1 at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 95-105% 98-104% 89-100%
EDTA plasma(n=10) 82-95% 85-94% 82-96%
Heparin plasma(n=10) 91-98% 84-100% 86-97%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/ml) 3.24 13.14 48.7 3.05 12.72 50.01
Standard deviation 0.16 0.64 2.35 0.14 0.69 2.54
CV(%) 4.95 4.89 4.82 4.69 5.43 5.08
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
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