Products

Human C5(Complement C5) ELISA Kit

EH0680 Catalogue No.
C5 ELISA Kit, Complement Component 5 ELISA Kit
Reactivity
Human
Range
3.125-200ng/ml
Sensitivity
1.875ng/ml
Product Name
Human C5(Complement C5) ELISA Kit
Catalogue No.
EH0680
Alias
C5 ELISA Kit, Complement Component 5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C5 ELISA Kit allows for the in vitro quantitative determination of C5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Human
UniProt ID
P01031
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of C5 and the recovery rates were calculated by comparing the measured value to the expected amount of C5 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)89-10495
EDTA plasma(n=5)89-10495
heparin plasma(n=5)91-10096
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)85-97%90-103%97-105%
EDTA plasma(n=5)93-101%85-98%83-97%
heparin plasma(n=5)82-89%84-100%81-94%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only