Products
Bovine Beta-LG(beta-lactoglobulin)ELISA kit
This kit is based on Competitive-ELISA detection method and takes 1h assay time. The microplate provided in this kit has been precoated with anti Beta-LG antibody. Sample or standard completely mixes with fixed amount of HRP labeled Beta-LG working solution and also add into each well. Beta-LG in the sample or standard competes with biotin-labeled Beta-LG to bind with antibody precoated on the microplate. Free components are washed off. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Beta-LG in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
- Catalogue No.:
- EB0185
- Alias:
- Beta-lactoglobulin ELISA Kit, Beta-LG ELISA Kit, Bos d 5 ELISA Kit, LGB ELISA Kit, Beta-LG ELISA Kit
- Species:
- Bovine
- Range:
- 0.156-10ug/ml
- Sensitivity:
- 0.094ug/ml
- SPECIFICATIONS
- Product Name
- Bovine Beta-LG(beta-lactoglobulin)ELISA kit
- Alias
- Beta-lactoglobulin ELISA Kit, Beta-LG ELISA Kit, Bos d 5 ELISA Kit, LGB ELISA Kit, Beta-LG ELISA Kit
- Catalogue No.
- EB0185
- Size
- 48T/96T
- Species
- Bovine
- UniProt No.
- P02754
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Competitive ELISA, Coated with antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 1 hours
- Range
- 0.156-10ug/ml
- Sensitivity
- 0.094ug/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with Beta-LG , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 HRP-Antigen(Concentrated, 100X) 30ul 60ul 2-8°C (Avoid Direct Light) E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antigen Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Step 1: Wash the plate twice before adding the standard and sample.
- Step 2: Add 100ul standard/sample/HRP-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37°C. (Equilibrate the whole bottle of TMB for 30min at 37°C.)
- Washing: Wash the plate five times and immerse for 1min each time.
- Step 3: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
- Step 4: Add 50ul stop solution. Read at 450nm immediately and calculate.
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ug/ml) OD-1 OD-2 Average 0 2.138 2.2 2.169 0.156 1.833 1.887 1.86 0.312 1.558 1.604 1.581 0.625 1.332 1.37 1.351 1.25 0.983 1.011 0.997 2.5 0.727 0.749 0.738 5 0.489 0.503 0.496 10 0.346 0.356 0.351 - Recovery
-
Add a certain amount of Beta-LG into the sample. Calculate the recovery by comparing the measured value with the expected amount of Beta-LG in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 92-105 97 EDTA plasma(n=10) 90-105 100 Heparin plasma(n=10) 87-103 96 - Linearity
-
Dilute the sample with a certain amount of Beta-LG at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 91-104% 84-96% 81-98% EDTA plasma(n=10) 86-103% 82-98% 83-96% Heparin plasma(n=10) 90-101% 87-101% 80-100% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ug/ml) 0.34 1.16 5.15 0.31 1.16 5.27 Standard deviation 0.02 0.06 0.25 0.01 0.05 0.27 CV(%) 4.93 5.22 4.89 4.82 4.68 5.12 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100