[Cited FineTest Protein] Current Research on Liver Injury Protection

FineTest protein contributes to the research on liver injury protection. The western blot is designed to measure HIF-1α in liver sample.

Publication Details
Article Title: A new class of potent liver injury protective compounds: Structural elucidation, total synthesis and bioactivity study
Journal Title: Acta Pharmaceutica Sinica B
DOI: 10.1016/j.apsb.2023.05.001
IF: 14.907

Abstract:    A new class of potent liver injury protective compounds, phychetins A−D (1−4) featuring an unique 6/6/5/6/5 pentacyclic framework, were isolated and structurally characterized from a Chinese medicinal plant Phyllanthus franchetianus. Compounds 2−4 are three pairs of enantiomers that were initially obtained in a racemic manner, and were further separated by chiral HPLC preparation. Compounds 1−4 were proposed to be originated biosynthetically from a coexisting lignan via an intramolecular Friedel‒Crafts reaction as the key step. A bioinspired total synthesis strategy was thus designated, and allowed the effective syntheses of compounds 2−4 in high yields. Some of compounds exhibited significant anti-inflammatory activities in vitro via suppressing the production of pro-inflammatory cytokine IL-1β. Notably, compound 4, the most active enantiomeric pair in vitro, displayed prominent potent protecting activity against liver injury at a low dose of 3 mg/kg in mice, which could serve as a promising lead for the development of acute liver injury therapeutic agent.

Keywords:  Phychetins, Phyllanthus franchetianus, Structural elucidation, Total synthesis, Liver injury protection

Western Blot

Validated Image

Figure Source: Acta Pharm Sin B. 2023. doi: 10.1016/j.apsb.2023.05.001.

Figure 4. an OPD assay was carried out using oligonucleotide spanning the HIF-1α binding site on the IL-1β promoter. Protein level was analyzed by Western blot (representative of n=2).