[Cited FineTest ELISA Kit] Current Research on Tumor Microenvironment

FineTest ELISA kit contributes to the research on tumor microenvironment. The immunoassay is designed to measure TGF-β1 concentration in monocytes-derived macrophages(M-DM).

Publication Details
Article Title: Exploring association of melanoma-specific Bcl-xL with tumor immune microenvironment
Journal Title: Journal of Experimental & Clinical Cancer Research
DOI: 10.1186/s13046-023-02735-9
IF: 11.3
PMID: 37488586

Abstract:   Macrophages take center stage in the tumor microenvironment, a niche composed of extracellular matrix and a heterogeneous group of cells, including immune ones. Human melanoma cells silencing or overexpressing Bcl-xL protein, THP-1 monocytic cells and monocyte-derived macrophages were used in this study. We demonstrated that melanoma cells overexpressing Bcl-xL recruit macrophages at the tumor site and induce a M2 phenotype. In addition, we identified that interleukin-8 and interleukin-1β cytokines are involved in macrophage polarization, and the chemokine CCL5/RANTES in the macrophages recruitment at the tumor site. We also found that all these Bcl-xL-induced factors are regulated in a NF-kB dependent manner in human and zebrafish melanoma models. Our findings confirmed the pro-tumoral function of Bcl-xL in melanoma through its effects on macrophage phenotype.

Keywords:   Bcl-xL, Melanoma, Tumor-associated macrophages

Immunoassay

Validated Image

Figure Source:  J Exp Clin Cancer Res. 2023 Jul 24;42(1):178. doi: 10.1186/s13046-023-02735-9.

Fig. 2 TGF-β protein secretion quantified by ELISA essay in M-DM .