[Cited FineTest ELISA Kit] Current Research on Stress-induced Hypertension

FineTest ELISA kit contributes to the research on stress-induced hypertension. The immunoassay is designed to measure norepinephrine(NE) concentration in plasma.

Publication Details
Article Title: CircRNA Galntl6 sponges miR-335 to ameliorate stress-induced hypertension through upregulating Lig3 in rostral ventrolateral medulla
Journal Title: Redox Biology
DOI: 10.1016/j.redox.2023.102782
IF: 10.787
PMID: 37315345

Abstract:  Rostral ventrolateral medulla (RVLM) is thought to serve as a major vasomotor center that participates in controlling the progression of stress-induced hypertension (SIH). Circular RNAs (circRNAs) perform important functions in the regulation of diverse physiological and pathological processes. However, information concerning the functions of RVLM circRNAs on SIH remains limited. RNA sequencing was performed to profile circRNA expression in RVLMs from SIH rats, which were induced by electric foot shocks and noises. The functions of circRNA Galntl6 in reducing blood pressure (BP) and its potential molecular mechanisms on SIH were investigated via various experiments, such as Western blot and intra-RVLM microinjection. A total of 12,242 circRNA transcripts were identified, among which circRNA Galntl6 was dramatically downregulated in SIH rats. The upregulation of circRNA Galntl6 in RVLM effectively decreased the BP, sympathetic outflow, and neuronal excitability in SIH rats. Mechanistically, circRNA Galntl6 directly sponged microRNA-335 (miR-335) and restrained it to reduce oxidative stress. Reintroduction of miR-335 observably reversed the circRNA Galntl6-induced attenuation of oxidative stress. Furthermore, Lig3 can be a direct target of miR-335. MiR-335 inhibition substantially increased the expression of Lig3 and suppressed oxidative stress, and these favorable effects were blocked by Lig3 knockdown. CircRNA Galntl6 is a novel factor that impedes SIH development, and the circRNA Galntl6/miR-335/Lig3 axis represents one of the possible mechanisms. These findings demonstrated circRNA Galntl6 as a possibly useful target for the prevention of SIH.

Keywords:  CircRNA Galntl6, Lig3, Rostral ventrolateral medulla, Stress-induced hypertension, miR-335

Immunoassay

Validated Image

Figure Source: Redox Biol. 2023 Jun 9;64:102782. doi: 10.1016/j.redox.2023.102782.

Fig. 2. Effects of circRNA Galntl6 overexpression on the sympathetic outflow were unveiled using RSNA recording test and plasma NE ELISA.