FineTest ELISA kit contributes to the research on stem cell therapy for rheumatoid arthritis. The immunoassay is designed to measure PGE2 concentration in supernatant.
Publication Details
Article Title: Infusion of GMSCs relieves autoimmune arthritis by suppressing the externalization of neutrophil extracellular traps via PGE2-PKA-ERK axis
Journal Title: Journal of Advanced Research
DOI: 10.1016/j.jare.2023.05.001
IF: 12.822
PMID: 37169220
Abstract: We found in this study that adoptive transfer of GMSCs into STIA mice significantly ameliorated experimental arthritis and reduced neutrophil infiltration and NET formation. In vitro studies also showed that GMSCs inhibited the generation of NETs in neutrophils. Subsequent investigations revealed that GMSCs secreted prostaglandin E2 (PGE2) to activate protein kinase A (PKA), which ultimately inhibited the downstream extracellular signal-regulated kinase (ERK) pathway that is essential for NET formation. Our results demonstrate that infusion of GMSCs can ameliorate inflammatory arthritis mainly by suppressing NET formation via the PGE2-PKA-ERK signaling pathway. These findings further support the notion that the manipulation of GMSCs is a promising stem cell-based therapy for patients with RA and other autoimmune and inflammatory diseases.
Keywords: Rheumatoid arthritis, gingival-derived mesenchymal stem cells, neutrophil extracellular traps, PGE2-PKA-ERK axis
Immunoassay
| FineTest Product | Sample | Detection Target |
| PGE2(Prostaglandin E2) ELISA Kit(EU2554) | supernatant | PGE2 |
Illustration
Figure Source: J Adv Res. 2023 May 9;S2090-1232(23)00127-3. doi: 10.1016/j.jare.2023.05.001.