[Cited FineTest ELISA Kit] Current Research on Neuroinflammation

FineTest ELISA kit contributes to the research on neuroinflammation. The immunoassay is designed to measure Hmgb1 concentration in plasma.

Publication Details
Article Title: FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation
Journal Title: Autophagy
DOI: 10.1080/15548627.2022.2116833
IF: 13.3
PMID: 36170234

Abstract:    Proper termination of cell-death-induced neural inflammation is the premise of tissue repair in acute ischemic stroke (AIS). Macrophages scavenge cell corpses/debris and produce inflammatory mediators that orchestrate immune responses. Here, we report that FOXP3, the key immune-repressive transcription factor of Tregs, is conditionally expressed in macrophages in stroke lesion. FOXP3 ablation in macrophages results in detrimental stroke outcomes, emphasizing the beneficial role of FOXP3+ macrophages. FOXP3+ macrophages are distinct from the M1 or M2 subsets and display superactive efferocytic capacity. With scRNAseq and analysis of FOXP3-bound-DNA isolated with CUT & RUN, we show that FOXP3 facilitates macrophage phagocytosis through enhancing cargo metabolism. FOXP3 expression is controlled by macroautophagic/autophagic protein degradation in resting macrophages, while initiation of LC3-associated phagocytosis (LAP) competitively occupies the autophagic machineries, and thus permits FOXP3 activation. Our data demonstrate a distinct set of FOXP3+ macrophages with enhanced scavenging capability, which could be a target in immunomodulatory therapy against AIS.

Keywords: FOXP3, neuroinflammation, macrophage, phagocytosis, stroke

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Figure Source:  Autophagy. 2023 Apr;19(4):1144-1163. doi: 10.1080/15548627.2022.2116833.