[Cited FineTest ELISA Kit] Current Research on Nanoparticles for Drug Delivery in Cancer Treatment

FineTest ELISA kit contributes to the research on nanoparticle drug delivery. The immunoassay is designed to measure PD-L1 concentration in αPD-L1-G5-R samples.

Publication Details
Article Title: High Throughput Screening of Surface Engineered Cyanine Nanodots for Active Transport of Therapeutic Antibody into Solid Tumor
Journal Title: Advanced Materials
DOI: 10.1002/adma.202302292
IF: 29.4
PMID: 37405862

Abstract: The successful delivery of therapeutic biomacromolecules into solid tumor holds great challenge due to their high resistance to penetrate through the complex tumor microenvironments. Here, we harness active-transporting nanoparticles to efficiently deliver biomacromolecular drugs into solid tumor through cell transcytosis. We prepared a serial of molecular precise cyanine 5-cored polylysine G5 dendrimers (Cy5 nanodots) with different peripheral amino acids (G5-AA). We evaluated the capability of these positive charged nanodots to induce cell endocytosis, exocytosis and transcytosis via fluorescence-based high throughput screen. The optimized nanodots (G5-R) was conjugated with αPD-L1 (a therapeutic monoclonal antibody binding to programmed-death ligand 1) (αPD-L1-G5-R) to demonstrate the nanoparticle-mediated tumor active transport. The αPD-L1-G5-R could greatly enhance the tumor penetration capability through adsorption-mediated transcytosis (AMT). We tested the effectiveness of αPD-L1-G5-R in treating mice bearing partially resected CT26 tumor, mimicking the local immunotherapy of residual tumors post-surgery in the clinic. The αPD-L1-G5-R embedded in fibrin gel could efficiently mediate tumor cell transcytosis, and deliver αPD-L1 throughout the tumor, thereby enhancing immune checkpoint blockade, reducing tumor recurrence, and significantly prolonging the survival time. The active transporting nanodots are promising platforms for efficient tumor delivery of therapeutic biomacromolecules.

Keywords: active transporting nanocarrier, dye-cored dendrimer, fluorescent nanodots, immune checkpoint blockade, nanoparticle drug delivery, transcytosis, tumor penetration

Immunoassay

Validated Image

Figure Source: Adv Mater. 2023 Jul 5;e2302292. doi: 10.1002/adma.202302292.

Figure S16. The activity ratio of αPD-L1-G5-R to αPD-L1. The αPD-L1-G5-R was prepared at αPD-L1-DBCO to G5-R-N3 molar ratio of 1:2, 1:5, or 1:10.