[Cited FineTest ELISA Kit] Current Research on Myocardial Ischemia–reperfusion Injury

FineTest ELISA kit contributes to the research on myocardial ischemia–reperfusion injury. The immunoassay is designed to measure Chi3l1 and BNP concentration in plasma.

Publication Details
Article Title: Single-cell profile reveals the landscape of cardiac immunity and identifies a cardio-protective Ym-1hi neutrophil in myocardial ischemia-reperfusion injury
Journal Title: Science Bulletin
DOI: 10.1016/j.scib.2024.02.003
IF: 18.9
PMID: 38395651

Abstract: Myocardial ischemia-reperfusion injury (MIRI) is a major hindrance to the success of cardiac reperfusion therapy. Although increased neutrophil infiltration is a hallmark of MIRI, the subtypes and alterations of neutrophils in this process remain unclear. Here, we performed single-cell sequencing of cardiac CD45+ cells isolated from the murine myocardium subjected to MIRI at six-time points. We identified diverse types of infiltrating immune cells and their dynamic changes during MIRI. Cardiac neutrophils showed the most immediate response and largest changes and featured with functionally heterogeneous subpopulations, including Ccl3^hi Neu and Ym-1^hi Neu, which were increased at 6 h and 1 d after reperfusion, respectively. Ym-1^hi Neu selectively expressed genes with protective effects and was, therefore, identified as a novel specific type of cardiac cell in the injured heart. Further analysis indicated that neutrophils and their subtypes orchestrated subsequent immune responses in the cardiac tissues, especially instructing the response of macrophages. The abundance of Ym-1^hi Neu was closely correlated with the therapeutic efficacy of MIRI when neutrophils were specifically targeted by anti-Lymphocyte antigen 6 complex locus G6D (Ly6G) or anti-Intercellular cell adhesion molecule-1 (ICAM-1) neutralizing antibodies. In addition, a neutrophil subtype with the same phenotype as Ym-1^hi Neu was detected in clinical samples and correlated with prognosis. Ym-1 inhibition exacerbated myocardial injury, whereas Ym-1 supplementation significantly ameliorated injury in MIRI mice, which was attributed to the tilt of Ym-1 on the polarization of macrophages toward the repair phenotype in myocardial tissue. Overall, our findings reveal the anti-inflammatory phenotype of Ym-1^hi Neu and highlight its critical role in myocardial protection during the early stages of MIRI.

Keywords: Cardiac immune response, Myocardial ischemia-reperfusion Injury, Neutrophil, Single-cell sequencing, Ym-1

Immunoassay

Validated Image

Figure Source: Sci Bull. 2024 Feb 7:S2095-9273(24)00072-0. doi: 10.1016/j.scib.2024.02.003.