[Cited FineTest ELISA Kit] Current Research on Magnetic Nanoparticles for Biomedical Applications

FineTest ELISA kit contributes to the research on magnetic nanoparticles for biomedical applications. The immunoassay is designed to measure GAGs concentration in Magcells.

Publication Details
Article Title: Sophisticated Magneto-Mechanical Actuation Promotes In Situ Stem Cell Assembly and Chondrogenesis for Treating Osteoarthritis
Journal Title: ACS Nano
DOI: 10.1021/acsnano.3c06909
IF: 17.1
PMID: 37862095

Abstract:  Abnormal mechanical loading often leads to the progressive degradation of cartilage and causes osteoarthritis (OA). Although multiple mechanoresponsive strategies based on biomaterials have been designed to restore healthy cartilage microenvironments, methods to remotely control the on-demand mechanical forces for cartilage repair pose significant challenges. Here, a magneto-mechanically controlled mesenchymal stem cell (MSC) platform, based on the integration of intercellular mechanical communication and intracellular mechanosignaling processes, is developed for OA treatment. MSCs loaded with antioxidative melanin@Fe₃O₄ magnetic nanoparticles (Magcells) rapidly assemble into highly ordered cell clusters with enhanced cell-cell communication under a time-varying magnetic field, which enables long-term retention and differentiation of Magcells in the articular cavity. Subsequently, via mimicking the gait cycle, chondrogenesis can be further enhanced by the dynamic activation of mechanical signaling processes in Magcells. This sophisticated magneto-mechanical actuation strategy provides a paradigm for developing mechano-therapeutics to repair cartilage in OA treatment.

Keywords: OA treatment, chondrogenesis, magnetic field, magneto-mechanical actuation, stem cell assembly

Immunoassay

Validated Image

Figure Source: ACS Nano. 2023 Oct 20. doi: 10.1021/acsnano.3c06909.
Figure 4. GAGs expression in Magcells treated with TGF-β under TMF stimulation or not for 14 days.