[Cited FineTest ELISA Kit] Current Research on Ischemia Reperfusion Injury

FineTest ELISA kit contributes to the research on ischemia reperfusion injury. The immunoassay is designed to measure OLFM4 concentration in small intestine.

Publication Details
Article Title: Microfluidic intestinal organoid-on-a-chip uncovers therapeutic targets by recapitulating oxygen dynamics of intestinal IR injury
Journal Title: Bioactive Materials
DOI: 10.1016/j.bioactmat.2023.07.001
IF: 18.9
PMID: 37534235

Abstract: Increasing evidence demonstrates that mammals have different reactions to hypoxia with varied oxygen dynamic patterns. It takes ∼24 h for tri-gas incubator to achieve steady cell hypoxia, which fails to recapitulate ultrafast oxygen dynamics of intestinal ischemia/reperfusion (IR) injury. Inspired from the structure of native intestinal villi, we engineered an intestinal organoid chip embedded with engineered artificial microvessels based on co-axial microfluidic technology by using pH-responsive ZIF-8/sodium alginate scaffold. The chip was featured on: (i) eight times the oxygen exchange efficiency compared with the conventional device, tri-gas incubator, (ii) implantation of intestinal organoid reproducing all types of intestinal epithelial cells, and (iii) bio-responsiveness to hypoxia and reoxygenation (HR) by presenting metabolism disorder, inflammatory reaction, and cell apoptosis. Strikingly, it was found for the first time that Olfactomedin 4 (Olfm4) was the most significantly down-regulated gene under a rapid HR condition by sequencing the RNA from the organoids. Mechanistically, OLFM4 played protective functions on HR-induced cell inflammation and tissue damage by inhibiting the NF-kappa B signaling activation, thus it could be used as a therapeutic target. Altogether, this study overcomes the issue of mismatched oxygen dynamics between in vitro and in vivo, and sets an example of next-generation multisystem-interactive organoid chip for finding precise therapeutic targets of IR injury.

Keywords: Ionic barrier of ZIF-8, Microfluidics, Organoid-on-a-chip, Oxygen dynamics, Olfm4

Immunoassay

Validated Image

Figure Source: Bioact Mater. 2023 Jul 21;30:1-14. doi: 10.1016/j.bioactmat.2023.07.001.