[Cited FineTest Elisa Kit] Current Research on Induced Pluripotent Stem Cells

FineTest Elisa kit contributes to the research on induced pluripotent stem cells. The immunoassay is designed to measure IGF-2 concentration in culture supernatant.

Publication Details
Article Title: Reconstitution of human adrenocortical specification and steroidogenesis using induced pluripotent stem cells
Journal Title: Developmental Cell
DOI: 10.1016/j.devcel.2022.10.010
IF: 13.417
PMID: 36413950

Abstract:   The mechanisms leading to adrenal cortex development and steroid synthesis in humans remain poorly understood due to the paucity of model systems. Herein, we recapitulate human fetal adrenal cortex specification processes through stepwise induction of human-induced pluripotent stem cells through posterior intermediate mesoderm-like and adrenocortical progenitor-like states to ultimately generate fetal zone adrenal-cortex-like cells (FZLCs), as evidenced by histomorphological, ultrastructural, and transcriptome features and adrenocorticotropic hormone (ACTH)-independent Δ5 steroid biosynthesis. Furthermore, FZLC generation is promoted by SHH and inhibited by NOTCH, ACTIVIN, and WNT signaling, and steroid synthesis is amplified by ACTH/PKA signaling and blocked by inhibitors of Δ5 steroid synthesis enzymes. Finally, NR5A1 promotes FZLC survival and steroidogenesis. Together, these findings provide a framework for understanding and reconstituting human adrenocortical development in vitro, paving the way for cell-based therapies of adrenal insufficiency.

Keywords: human-induced pluripotent stem cells, NR5A1, adrenal capsule, adrenal cortex, adrenocortical development, adrenocorticotropic hormone, definitive zone, fetal zone, intermediate mesoderm, steroidogenesis

Immunoassay

Validated Image

Figure Source:  Dev Cell. 2022 Nov 21;57(22):2566-2583.e8. doi: 10.1016/j.devcel.2022.10.010.

Figure 2. Induction of fetal-zone adrenocortical-like cells (FZLCs) through air-liquid interface culture.