High IF Citations

[Cited FineTest Elisa Kit] Current Research on Gut Health and Aging

FineTest Elisa kit contributes to the research on gut health and aging. The immunoassay is designed to measure IL-10 concentration in supernatant and serum.

Publication Details
Article Title: Dietary genistein increases microbiota-derived short chain fatty acid levels, modulates homeostasis of the aging gut, and extends healthspan and lifespan
Journal Title: Pharmacological Research
DOI: 10.1016/j.phrs.2023.106676
IF: 10.334
PMID: 36693599

Abstract:       Age-related gastrointestinal decline contributes to whole-organism frailty and mortality. Genistein is known to have beneficial effects on age-related diseases, but its precise role in homeostasis of the aging gut remains to be elucidated. Here, wild-type aging mice and Zmpste24-/- progeroid mice were used to investigate the role of genistein in lifespan and homeostasis of the aging gut in mammals. A series of longitudinal, clinically relevant measurements were performed to evaluate the effect of genistein on healthspan. It was found that dietary genistein promoted a healthier and longer life and was associated with a decrease in the levels of systemic inflammatory cytokines in aging mice. Furthermore, dietary genistein ameliorated gut dysfunctions, such as intestinal inflammation, leaky gut, and impaired epithelial regeneration. A distinct genistein-mediated alteration in gut microbiota was observed by increasing Lachnospira abundance and short-chain fatty acid (SCFA) production. Further fecal microbiota transplantation and dirty cage sharing experiments indicated that the gut microbiota from genistein-fed mice rejuvenated the aging gut and extended the lifespan of progeroid mice. It was demonstrated that genistein-associated SCFAs alleviated tumor necrosis factor alpha-induced intestinal organoid damage. Moreover, genistein-associated propionate promoted regulatory T cell-derived interleukin 10 production, which alleviated macrophage-derived inflammation. This study provided the first data, to the authors’ knowledge, indicating that dietary genistein modulates homeostasis in the aging gut and extends the healthspan and lifespan of aging mammals. Moreover, the existence of a link between genistein and the gut microbiota provides a rationale for dietary interventions against age-associated frailty.

Keywords:    Aging gut, Genistein, Healthspan, Lachnospira, Short-chain fatty acid, Progeroid mice

Immunoassay

FineTest Product Sample Detection Target Species
Mouse IL-10(Interleukin-10) ELISA Kit(EM0100) supernatant/serum IL-10 Mouse

Validated Image

aging

Figure Source:  Pharmacol Res. 2023 Jan 21;188:106676. doi: 10.1016/j.phrs.2023.106676.

Fig. 4. Expression of cytokine (IL-10) of the colon, (n = 5).