FineTest ELISA kit contributes to the research on gastric cancer treatment. The immunoassay is designed to measure FAM3c concentration in conditioned mediums.
Publication Details
Article Title: Neutrophils promote tumor invasion via FAM3C-mediated epithelial-to-mesenchymal transition in gastric cancer
Journal Title: International Journal of Biological Sciences
DOI: 10.7150/ijbs.79022
IF: 10.75
Abstract: In gastric cancer, lymph node metastasis (LNM) is the major metastasis route, and lymphatic invasion is the precursor of LNM. Tumor-associated neutrophils (TANs) promote LNM. However, the molecular mechanisms underlying TANs-mediated lymphatic invasion and/or LNM remain unclear. Herein, we revealed that high level of TANs was the independent risk factor for lymphatic invasion and LNM respectively, and lymphatic tumor cell-neutrophil clusters were positively correlated with LNM. Crosstalk between neutrophils and tumor cells was required for enhanced tumor cell invasiveness, endowing neutrophils to boost epithelial-to-mesenchymal transition (EMT) of tumor cells and in turn promoting LNM. Mechanically, tumor cells educated neutrophils via TGFβ1 to produce more FAM3C through Smad2/3 signaling activation, and FAM3C promoted tumor cell EMT through JNK-ZEB1/Snail signaling pathway. The crosstalk enhanced the affinity of neutrophils with tumor cells through interaction of integrins α6β1 and α6β4 with CD151. Furthermore, studies using tumor-bearing mice demonstrated that neutrophils were the important driver for gastric cancer tumorigenesis and invasiveness. The study clearly identifies the functional roles of TANs in promoting tumor invasion, and facilitates a better understanding of novel mechanisms responsible for LNM of gastric cancer, which provides potential targets for developing new strategies to prevent or treat LNM in gastric cancer.
Keywords: Gastric cancer treatment, Tumor-associated neutrophils, Lymph node metastasis, Epithelial-to-mesenchymal transformation, FAM3C
Immunoassay
FineTest Product | Sample | Detection Target | Species |
Human FAM3c(Protein FAM3c) ELISA Kit(EH4449) | conditioned mediums | FAM3c | Human |
Validated Image
Figure Source: Int J Biol Sci 2023; 19(5):1352-1368. doi:10.7150/ijbs.79022.
Figure 3C. Co-culture with MKN28 or MKN45 cells increased FAM3C level.