[Cited FineTest Elisa Kit] Current Research on Dengue Fever Treatment

FineTest Elisa kit contributes to the research on dengue fever treatment. The immunoassay is designed to measure PAR-1 concentration in serum.

Publication Details
Article Title: Elevated serum PAR‐1 levels as an emerging biomarker of inflammation to predict the dengue infection severity
Journal Title: Journal of Medical Virology
DOI: 10.1002/jmv.28152
IF: 20.693
PMID: 36109338

Abstract:     The present study was designed to check the serum levels of protease-activated receptor (PAR-1) in patients during different phases of dengue severity. Moreover, a correlation between serum PAR-1 levels and hematological parameters, inflammatory cytokine levels, and liver functional changes was also determined. Based on the World Health Organization criteria, the study population was divided into: nonsevere dengue fever (DF; n = 30), severe dengue hemorrhagic fever (DHF; n = 19), and severe dengue shock syndrome (DSS; n = 11). The platelet count (PLT) and hematocrit (HCT) were analyzed using an automated hematology analyzer and liver function enzymes aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphate (ALP), bilirubin were checked by auto-analyzer using diagnostic kits. Moreover, the levels of inflammatory mediators C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-17 (IL-17), and PAR-1 were determined using respective ELISA kits. The HCT levels were elevated and platelet count decreased significantly during dengue complications (DHF and DSS) compared to the DF patients, while the levels of liver functional biomarkers AST, ALT, ALP, and bilirubin remained elevated in DHF and DSS groups than in the corresponding DF group. Similarly, the inflammatory cytokine levels of CRP, TNF-α, IL-6, and IL-17 in DHF and DSS subjects were markedly increased when observed against DF subjects. Notably, the PAR-1 levels were significantly elevated in DHF and DSS groups than in the DF group and positively correlated with changes in HCT levels, inflammatory biomarkers, and liver enzymes. Our findings conclude that PAR-1 levels persistently increased with the severity of the dengue infection and are strongly associated with various clinical manifestations. Thus, PAR-1 levels can be used as a diagnostic marker for assessing dengue severity.
Keywords:    dengue; inflammation; liver dysfunction; protease activated receptor-1; shock

Immunoassay

Validated Image

Figure Source: J Med Virol. 2022 Sep 15. doi: 10.1002/jmv.28152.

Figure 2.  Panels [A] and [B] show statistical comparison of serum PAR-1 levels in primary and secondary infection groups of DHF and DSS patients. *p<0.05 vs primary infection group respectively.