[Cited FineTest ELISA Kit] Current Research on Cancer Associated Fibroblasts in Tumor Microenvironment

FineTest ELISA kit contributes to the research on cancer associated fibroblasts in tumor microenvironment. The immunoassay is designed to measure HMGB1 level in cell supernatants.

Article Title: Spatial Regulation of Cancer-Associated Fibroblasts and Tumor Cells via pH-Responsive Bispecific Antibody Delivery for Enhanced Chemo-Immunotherapy Synergy
Journal Title: ACS Nano
DOI: 10.1021/acsnano.4c13277
IF: 15.8
PMID: 40114589

Abstract:   The effectiveness of chemotherapy is often compromised by physiological barriers and an immunosuppressive tumor microenvironment. Cancer-associated fibroblasts (CAFs) significantly contribute to the reconfiguration of the tumor extracellular matrix (ECM) and the suppression of immune responses, making them crucial targets for therapeutic intervention. Here, a tumor acidic microenvironment-responsive delivery system that utilizes tumor cell-derived microparticles (MPs) as carriers for the chemotherapeutic agent doxorubicin (DOX) and the bispecific antibody YM101 targeting both TGF-β and PD-L1 is developed (DOX@MPs-YM101) to spatially regulate both CAFs and tumor cells for enhanced chemotherapeutic efficacy. DOX@MPs-YM101 efficiently targets tumor tissues and releases DOX@MPs and YM101 in response to the acidic tumor microenvironment. YM101 reprograms CAFs and reduces the tumor ECM, facilitating tumor accumulation and deep penetration of DOX@MPs-YM101. DOX@MPs are highly internalized into tumor cells, triggering immunogenic cell death (ICD) and activating CD8+ T cell-mediated antitumor immunity. The reprogramming of CAFs by YM101 further promotes the accumulation of CD8+ T cells and reduces the number of immunosuppressive cells within the tumors. Additionally, YM101 effectively neutralizes PD-L1 on tumor cells induced by DOX@MPs, restoring CD8+ T cell activity and generating long-term antitumor immune memory to prevent tumor recurrence. Our findings highlight the potential of DOX@MPs-YM101 to improve chemotherapy in cancer treatment.

Keywords:  bispecific antibody, cancer-associated fibroblasts, chemo-immunotherapy, immunogenic cell death, tumor cell-derived microparticles

Immunoassay

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Figure Source: ACS Nano. 2025 Apr 1;19(12):11756-11773. doi: 10.1021/acsnano.4c13277.