[Cited FineTest Elisa Kit] Current Research on Biomaterials for Bone Tissue Engineering

FineTest Elisa kit contributes to the research on biomaterials for bone tissue engineering. The immunoassay is designed to measure SEMA7A concentration in supernatant.

Publication Details
Article Title: Construction of a Hierarchical Micro-/Submicro-/Nanostructured 3D-Printed Ti6Al4V Surface Feature to Promote Osteogenesis: Involvement of Sema7A through the ITGB1/FAK/ERK Signaling Pathway
Journal Title: ACS Applied Materials & Interfaces
DOI: 10.1021/acsami.2c06454
IF: 10.383
PMID: 35776897

Abstract: Constructing hierarchical hybrid structures is considered a facile method to improve the osseointegration of implants. Herein, a hierarchical micro-/submicro-/nanostructured surface feature of Ti6Al4V implants (3DAT group) was successfully constructed by combining the inherently formed three-dimensional (3D)-printed microscale topography, acid-etched sub-micropits, and anodized nanotubes. Compared with the classical SLA surface, the microscale topography and sub-micropits increased the three-dimensional space for the cell growth and mechanical stability of implants, while the modification of nanotubes dramatically improved the surface hydrophilicity, protein adsorption, and biomineralization. Most importantly, the 3DAT surface feature possessed excellent osteogenic performance in vitro and in vivo, with the involvement of semaphorin 7A (Sema7A) as revealed by RNA-seq through the ITGB1/FAK/ERK signaling pathway. The present study suggested that the hierarchically structured surface design strategy could accelerate the osseointegration rate of 3D-printed Ti6Al4V implants, promising personalized reconstruction of bone defects.

Keywords: 3D printing, titanium implant, hierarchical structure modification, osteogenesis, semaphorin 7A, biomaterials

Immunoassay

FineTest Product Sample Detection Target Species
Rat SEMA7A(Semaphorin-7A) ELISA Kit (ER1331) Supernatant SEMA7A Rat

Validated Image

biomaterials

Figure Source: ACS Appl Mater Interfaces. 2022 Jul 13;14(27):30571-30581. doi: 10.1021/acsami.2c06454.

Figure S5. The gene knockdown (A) and overexpression (B) efficiency of si-Sema7a and Lv-OE.