FineTest Elisa kit contributes to the research on mental disorder. The immunoassay is designed to measure LETM1, SNPH and SARM1 concentration in plasma.
Publication Details
Article Title: Abnormal Levels of Mitochondrial Proteins in Plasma Neuronal Extracellular Vesicles in Major Depressive Disorder
Journal Title: Molecular Psychiatry
DOI: 10.1038/s41380-021-01268-x
IF: 15.992
PMID: 34471251
Abstract: To characterize neuronal mitochondrial abnormalities in major depressive disorder (MDD), functional mitochondrial proteins (MPs) extracted from enriched plasma neuron-derived extracellular vesicles (NDEVs) of MDD participants (n = 20) were quantified before and after eight weeks of treatment with a selective serotonin reuptake inhibitor (SSRI). Pretreatment baseline NDEV levels of the transcriptional type 2 nuclear respiratory factor (NRF2) which controls mitochondrial biogenesis and many anti-oxidant gene responses, regulators of diverse neuronal mitochondrial functions cyclophilin D (CYPD) and mitofusin-2 (MFN2), leucine zipper EF-hand containing transm embrane 1 protein (LETM1) component of a calcium channel/calcium channel enhancer, mitochondrial tethering proteins syntaphilin (SNPH) and myosin VI (MY06), inner membrane electron transport complexes I (subunit 6) and III (subunit 10), the penultimate enzyme of nicotinamide adenine dinucleotide (NAD) generation nicotinamide mononucleotide adenylytransferase 2 (NMNAT2), and neuronal mitochondrial metabolic regulatory and protective factors humanin and mitochondrial open-reading frame of the 12S rRNA-c (MOTS-c) all were significantly lower than those of NDEVs from matched controls (n = 10), whereas those of pro-neurodegenerative NADase Sterile Alpha and TIR motif-containing protein 1 (SARM1) were higher. The baseline NDEV levels of transcription factor A mitochondrial (TFAM) and the transcriptional master-regulator of mitochondrial biogenesis PPAR γ coactivator-1α (PGC-1α) showed no differences between MDD participants and controls. Several of these potential biomarker proteins showed substantially different changes in untreated MDD than those we reported in untreated first-episode psychosis. NDEV levels of MPs of all functional classes, except complex I-6, NRF2 and PGC-1α were normalized in MDD participants who responded to SSRI therapy (n = 10) but not in those who failed to respond (n = 10) by psychiatric evaluation. If larger studies validate NDEV MP abnormalities, they may become useful biomarkers and identify new drug targets.
Keywords: Calcium-Binding Proteins, Depressive Disorder, Extracellular Vesicles, Mitochondrial Proteins, Nerve Tissue Proteins, Neurons, Mental Disorder
Immunoassay
| FineTest Product | Sample | Detection Target | Species |
| Human SNPH(Syntaphilin)ELISA Kit (EH12769) | Plasma | SNPH | Human |
| Human LETM1 ELISA Kit (EH9771) | LETM1 | Human | |
| Human SARM1(Sterile alpha and TIR motif-containing protein 1) ELISA Kit (EH12072) | SARM1 | Human |
Validated Images
Figure Source: Mol Psychiatry, 2021 Dec;26(12):7355-7362. doi: 10.1038/s41380-021-01268-x
Fig. 1. NDEV levels of proteins involved in mitochondrial dynamics and other maintenance functions: 1676 ± 97 pg/ml, 758 ± 188 pg/ml (0.0004), 440 ± 44 pg/ml (NS), 523 ± 60 pg/ml (<0.0001) and 1797 ± 283 pg/ml (0.0008) for SNPH (E); and 2275 ± 221 pg/ ml, 1212 ± 169 pg/ml (0.0012), 796 ± 103 pg/ml (0.0412), 860 ± 114 pg/ml (<0.0001) and 2197 ± 248 pg/ml (0.0004) for LETM-1 (G).
Figure Source: Mol Psychiatry, 2021 Dec;26(12):7355-7362. doi: 10.1038/s41380-021-01268-x
Fig. 2. NDEV levels of proteins involved in mitochondrial generation of energy: 466 ± 74 pg/ml, 2063 ± 351 pg/ml (0.0002), 2695 ± 1047 pg/ml (NS), 1908 ± 416 pg/ml (0.0031) and 664 ± 156 pg/ml (0.0368) for SARM1 (D).
Table 3. Comparison of alterations in NDEV levels of mitochondrial proteins in untreated first episode of psychosis (FP) and in untreated baseline for major depressive disorder (MDD).
Table Source: Mol Psychiatry, 2021 Dec;26(12):7355-7362. doi: 10.1038/s41380-021-01268-x