FineTest Elisa kit contributes to the research on mast cell and blood circulation. The immunoassay is designed to measure chymase 1 (Cma-1) concentration in serum.
Publication Details
Article Title: Directional Mast Cell Degranulation of Tumor Necrosis Factor into Blood Vessels Primes Neutrophil Extravasation
Journal Title: Immunity
DOI: 10.1016/j.immuni.2020.12.017
IF: 22.553
PMID: 33484643
Abstract: Tissue resident mast cells (MCs) rapidly initiate neutrophil infiltration upon inflammatory insult, yet the molecular mechanism is still unknown. Here, we demonstrated that MC-derived tumor necrosis factor (TNF) was crucial for neutrophil extravasation to sites of contact hypersensitivity-induced skin inflammation by promoting intraluminal crawling. MC-derived TNF directly primed circulating neutrophils via TNF receptor-1 (TNFR1) while being dispensable for endothelial cell activation. The MC-derived TNF was infused into the bloodstream by directional degranulation of perivascular MCs that were part of the vascular unit with access to the vessel lumen. Consistently, intravenous administration of MC granules boosted neutrophil extravasation. Pronounced and rapid intravascular MC degranulation was also observed upon IgE crosslinking or LPs challenge indicating a universal MC potential. Consequently, the directional MC degranulation of pro-inflammatory mediators into the bloodstream may represent an important target for therapeutic approaches aimed at dampening cytokine storm syndromes or shock symptoms, or intentionally pushing immune defense.
Keywords: blood vessel, contact hypersensitivity, degranulation, extravasation, inflammation, mast cell, neutrophil, tumor necrosis factor
Immunoassay
| FineTest Product | Sample | Detection Target | Species |
| Mouse Cma1(Chymase 1, Mast Cell) ELISA Kit (EM0717) | Serum | Cma-1 | Mouse |
Validated Image
Figure Source: Immunity, 2021 Mar 9;54(3):468-483.e5. doi: 10.1016/j.immuni.2020.12.017.
Figure 7. Intravascular MC degranulation quantitative evaluation in different settings of skin inflammation: (B and C) Cma-1 (B) and TNF (C) was quantified in serum samples from MCDTnf mice or MCWT controls isolated at the indicated time points post DNFB. (n = 8-14 for DNFB samples, n = 4 for veh controls; *p < 0.001 versus veh; **p < 0.05 versus MCWT).