【Cited FineTest Antibody】 Current Research on Haematological Cancer

FineTest antibody contributes to the research on haematological cancer. The secondary antibody is used to perform immunofluorescence.

Publication Details
Article Title: Inhibition of Cystathionine β-synthase Promotes Apoptosis and Reduces Cell Proliferation in Chronic Myeloid Leukemia
Journal Title: Signal Transduction and Targeted Therapy
DOI: 10.1038/s41392-020-00410-5
IF: 18.187
PMID: 33558454

Abstract:   Increased endogenous hydrogen sulfide (H2S) level by cystathionine β-synthase (CBS) has been shown to closely relate tumorigenesis. H2S promotes angiogenesis, stimulates bioenergy metabolism and inhibits selective phosphatases. However, the role of CBS and H2S in chronic myeloid leukemia (CML) remains elusive. In this study, we found that CBS and H2S levels were increased in the bone marrow mononuclear cells of pediatric CML patients, as well as in the CML-derived K562 cells and CBS expression levels were correlated with different disease phases. Inhibition of CBS reduced the proliferation of the CML primary bone marrow mononuclear cells and induced growth inhibition, apoptosis, cell cycle arrest, and migration suppression in K562 cells and tumor xenografts. The knockdown of CBS expression by shRNA and inhibiting CBS activity by AOAA decreased the endogenous H2S levels, promoted mitochondrial-related apoptosis and inhibited the NF-κB-mediated gene expression. Our study suggests that inhibition of CBS induces cell apoptosis, as well as limits cell proliferation and migration, a potential target for the treatment of chronic myeloid leukemia.

Keywords: Apoptosis, Bone Marrow Cells, Carcinogenesis, Cystathionine beta-Synthase, Energy Metabolism, Neovascularization, Haematological Cancer

Immunofluorescence