anti- HIF1a antibody
protein A+G purified
≥95% as determined by SDS-PAGE
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 24 months(Avoid repeated freeze / thaw cycles.)
Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).
hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
BHLHE78, MOP1, PASD8
ELISA, IF, WB
WB: 1:1000-1:10000; IF: 1:50-1:200
anti- HIF1a antibody 1
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab09940(HIF1a Antibody) at dilution of 1:5000
Current product's manual: Manual
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