Products

HIF1a antibody

Synonyms:BHLHE78 antibody, MOP1 antibody, PASD8 antibody
Catalogue No.:FNab09940Reativity:Human
Host:MouseTested Application:ELISA, IF, WB
Clonality:monoclonalIsotype:IgG1
  • SPECIFICATIONS
Product Name
HIF1a antibody
Catalogue No.
FNab09940
Size
100μg
Form
liquid
Purification
protein A+G purified
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG1
Clone ID
5H12
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
Alternative Names
BHLHE78 antibody, MOP1 antibody, PASD8 antibody
UniProt ID
Q16665
Observed MW
120 kDa
Application
Tested Applications
ELISA, IF, WB
Recommended dilution
WB: 1:1000-1:10000; IF: 1:50-1:200
Validated Images
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab09940(HIF1a Antibody) at dilution of 1:5000
Immunofluorescent analysis of ( -20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using FNab09940 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L)
Background
Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).