Flow Cytometry Services
FineTest® - Professional FCM Colour Matching Services
FineTest® - Professional FCM Colour Matching Services
We provide professional and free colour matching services to customers based on their experimental requirements. Only targets and configuration of flow cytometer are required by our technical team, like laser, detection channel, optical filters etc.
FineTest® - Professional FCM Data Analysis Services
FineTest® - Professional FCM Data Analysis Services
Customers using FineTest® FCM products can send the original data in FCS format and logic relationship of targets to our technical team. We can provide professional data analysis.
Advantages
Advantages
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High Quality:
Derived from classically cloned and excellently labelling technology
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Various Dyes:
High-performance in multicolour flow cytometry
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Complete Solution:
Free colour matching, assay guidance and data analysis services
Types of Dyes:
Types of Dyes:
Over 10 dyes for multicolour flow cytometry
Flow Cytometry Colour Matching for Immunocyte Subpopulations
Flow Cytometry Colour Matching for Immunocyte Subpopulations
| Species | Cell Populations | Flow Cytometry Antibody Combination | Cat.No |
| Human | T/B/NK cell populations detection | CD45-PerCP | PCP-30039 |
| CD3-FITC | FITC-30004 | ||
| CD16-PE | PE-30061 | ||
| CD56-PE | PE-30008 | ||
| CD19-APC | APC-30066 | ||
| Human | Thl/Th2 cell populations detection | CD3-PerCP/Cyanine5.5 | PCP55-30004 |
| CD4-FITC | FITC-30005 | ||
| IFN-γ-PE | PE-30053 | ||
| IL4-APC | APC-30043 | ||
| Mouse | Thl/Th2 cell populations detection | CD3-PerCP/Cyanine5.5 | PCP55-30002 |
| CD4-FITC | FITC-30001 | ||
| IFN-γ-PE | PE-30074 | ||
| IL4-APC | APC-30026 | ||
| Human | Treg cell populations detection | CD4-FITC | FITC-30005 |
| CD25-PE | PE-30035 | ||
| CD127-APC | APC-30033 | ||
| Mouse | Treg cell populations detection | CD4-FITC | FITC-30001 |
| CD25-PE | PE-30017 | ||
| FOXP3-APC | APC-30055 |
FCM Colour Matching and Human Detection Cases
FCM Colour Matching and Human Detection Cases
Case 1: T Cell Detection in Human Blood
| Target | Fluorescein | Clone ID | Cat.No |
|---|---|---|---|
| CD3 | FITC | OKT-3 | FITC-30004 |
| CD4 | PE | RPA-T4 | PE-30005 |
| CD8 | APC | OKT-8 | APC-30006 |
tips:
- ① Obvious grouping for CD3/CD4/CD8 without isotype control.
- ② Red blood cell lysis is very important. Excessive or insufficient lysis can lead to obscure grouping of lymphocyte.
Case 2: TH1 Cell Detection in Human Blood
| Target | Fluorescein | Clone ID | Cat.No |
|---|---|---|---|
| CD3 | FineTest®647 | OKD-3 | F647-30004 |
| CD4 | FITC | RPA-T4 | FITC-30005 |
| IFN-γ | PE | B27 | PE-30053 |
tips:
- ① Obvious grouping for CD3/CD4 without isotype control. IFN-γ detection requires for isotype control.
- ② Stimulation of PMA can cause endocytosis of CD4+T cell epitope. CD4 antibody(Clone ID: RPA-T4) can reduce endocytic effects of CD4 epitope via detecting permeabilized CD4 epitope. Grouping of CD4 cell is clearer.
- ③ As the cytokine, IFN-γ should be detected after blocking, fixation and permeabilization.
Case 3: Treg Cell Detection in Human Blood
| Target | Fluorescein | Clone ID | Cat.No |
|---|---|---|---|
| CD3 | PerCP/Cyanine5.5 | OKT-3 | PCP55-30004 |
| CD4 | FITC | RPA-T4 | FITC-30005 |
| CD25 | PE | BC96 | PE-30035 |
| CD127 | FineTest®647 | A019D5 | F647-30033 |
tips:
- ① Detection steps for FOXP3 in human blood is complex. It's not easy to detect FOXP3 as well. Human Treg cell can be detected with CD127. The ratio of CD4+CD25+CD127-/low is equivalent to the ratio of Treg cell.
- ② Setting of single positive tube is required for regulating compensation.
FCM Colour Matching and Mouse Detection Cases
FCM Colour Matching and Mouse Detection Cases
Case 1: T Cell Detection in Mouse Spleen
| Target | Fluorescein | Clone ID | Cat.No |
|---|---|---|---|
| CD3 | FITC | 17A2 | FITC-30127 |
| CD4 | PE | RM4-5 | PE-30128 |
| CD8 | APC | 53-6.7 | APC-30003 |
tips:
- ① Obvious grouping for CD3/CD4/CD8 without isotype control.
- ② Red blood cell lysis is very important. Excessive or insufficient lysis can lead to obscure grouping of lymphocyte.
Case 2: TH1 Cell Detection in Mouse Spleen
| Target | Fluorescein | Clone ID | Cat.No |
|---|---|---|---|
| CD3 | FineTest®647 | 17A2 | F647-30127 |
| CD4 | FITC | RM4-5 | FITC-30128 |
| IFN-γ | PE | XMG1.2 | PE-30074 |
tips:
- ① Obvious grouping for CD3/CD4 without isotype control. IFN-γ detection requires for isotype control.
- ② Stimulation of PMA can cause endocytosis of CD4+T cell epitope. CD4 antibody(Clone ID: RM4-5) can reduce endocytic effects of CD4 epitope via detecting permeabilized CD4 epitope. Grouping of CD4 cell is clearer.
- ③ As the cytokine, IFN-γ should be detected after blocking, fixation and permeabilization.
Case 3: Treg Cell Detection in Mouse Spleen
| Target | Fluorescein | Clone ID | Cat.No |
|---|---|---|---|
| CD4 | FITC | RM4-5 | FITC-30128 |
| CD25 | APC | PC-61.5.3 | APC-30017 |
| FOXP3 | PE | 3G3 | PE-30111 |
tips:
- ① Obvious grouping for CD4 without isotype control. Detection of CD25 and FOXP3 requires for isotype control.
- ② FOXP3 can’t be detected using improper fixation and permeabilization kit. K081(Foxp3/Transcription Factor Staining Kit) is recommended.
