[Cited FineTest Protein] Current Research on Bacterial Sepsis Treatment

FineTest protein contributes to the research on bacterial sepsis treatment. The immunoblot is designed to measure SIRT2 in BMDMs and Raw 264.7 cell lysates.

Publication Details
Article Title: A metabolite from commensal Candida albicans enhances the bactericidal activity of macrophages and protects against sepsis
Journal Title: Cellular & Molecular Immunology
DOI: 10.1038/s41423-023-01070-5
IF: 24.1
PMID: 37553429

Abstract: The gut microbiome is recognized as a key modulator of sepsis development. However, the contribution of the gut mycobiome to sepsis development is still not fully understood. Here, we demonstrated that the level of Candida albicans was markedly decreased in patients with bacterial sepsis, and the supernatant of Candida albicans culture significantly decreased the bacterial load and improved sepsis symptoms in both cecum ligation and puncture (CLP)-challenged mice and Escherichia coli-challenged pigs. Integrative metabolomics and the genetic engineering of fungi revealed that Candida albicans-derived phenylpyruvate (PPA) enhanced the bactericidal activity of macrophages and reduced organ damage during sepsis. Mechanistically, PPA directly binds to sirtuin 2 (SIRT2) and increases reactive oxygen species (ROS) production for eventual bacterial clearance. Importantly, PPA enhanced the bacterial clearance capacity of macrophages in sepsis patients and was inversely correlated with the severity of sepsis in patients. Our findings highlight the crucial contribution of commensal fungi to bacterial disease modulation and expand our understanding of the host-mycobiome interaction during sepsis development.

Keywords: Bacterial clearance, Candida albicans, Macrophage, Phenylpyruvate, Sepsis

Immunoblot

Validated Image

Figure Source: Cell Mol Immunol. 2023 Aug 9. doi: 10.1038/s41423-023-01070-5.

Fig. 5 Immunoblot analysis of SIRT2 in pronase-digested (B) BMDMs and (C) Raw 264.7 cell lysates (n = 3).