[Cited FineTest ELISA Kit] Current Research on Type 2 Inflammation Treatment

FineTest ELISA kit contributes to the research on type 2 inflammation treatment. The immunoassay is designed to measure CXCL2 level in cell supernatant.

Article Title: Alarmin-loaded extracellular lipid droplets induce airway neutrophil infiltration during type 2 inflammation
Journal Title: Immunity
DOI: 10.1016/j.immuni.2024.09.001
IF: 25.5
PMID: 39366382

Abstract: Group 2 innate lymphoid cells (ILC2s) play a crucial role in allergic diseases by coordinating a complex network of various effector cell lineages involved in type 2 inflammation. However, their function in regulating airway neutrophil infiltration, a deleterious symptom of severe asthma, remains unknown. Here, we observed ILC2-dependent neutrophil accumulation in the bronchoalveolar lavage fluid (BALF) of allergic mouse models. Chromatography followed by proteomics analysis identified the alarmin high mobility group box-1 (HMGB1) in the supernatant of lung ILC2s initiated neutrophil chemotaxis. Genetic perturbation of Hmgb1 in ILC2s reduced BALF neutrophil numbers and alleviated airway inflammation. HMGB1 was loaded onto the membrane of lipid droplets (LDs) released from activated lung ILC2s. Genetic inhibition of LD accumulation in ILC2s significantly decreased extracellular HMGB1 abundance and BALF neutrophil infiltration. These findings unveil a previously uncharacterized extracellular LD-mediated immune signaling delivery pathway by which ILC2s regulate airway neutrophil infiltration during allergic inflammation.

Keywords: extracellular lipid droplets, alarmin HMGB1, group 2 innate lymphoid cells, neutrophil infiltration, type 2 inflammation

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Figure Source: Immunity. 2024 Sep 25:S1074-7613(24)00445-X. doi: 10.1016/j.immuni.2024.09.001.