[Cited FineTest ELISA Kit] Current Research on Oncometabolism

FineTest ELISA kit contributes to the research on oncometabolism. The immunoassay is designed to measure ENO2 concentration in serum.

Publication Details
Article Title: ENO2-derived phosphoenolpyruvate functions as an endogenous inhibitor of HDAC1 and confers resistance to antiangiogenic therapy
Journal Title: Nature Metabolism
DOI: 10.1038/s42255-023-00883-y
IF: 20.8
PMID: 37667133

Abstract: Metabolic reprogramming is associated with resistance to antiangiogenic therapy in cancer. However, its molecular mechanisms have not been clearly elucidated. Here, we identify the glycolytic enzyme enolase 2 (ENO2) as a driver of resistance to antiangiogenic therapy in colorectal cancer (CRC) mouse models and human participants. ENO2 overexpression induces neuroendocrine differentiation, promotes malignant behaviour in CRC and desensitizes CRC to antiangiogenic drugs. Mechanistically, the ENO2-derived metabolite phosphoenolpyruvate (PEP) selectively inhibits histone deacetylase 1 (HDAC1) activity, which increases the acetylation of β-catenin and activates the β-catenin pathway in CRC. Inhibition of ENO2 with enolase inhibitors AP-III-a4 or POMHEX synergizes the efficacy of antiangiogenic drugs in vitro and in mice bearing drug-resistant CRC xenograft tumours. Together, our findings reveal that ENO2 constitutes a useful predictive biomarker and therapeutic target for resistance to antiangiogenic therapy in CRC, and uncover a previously undefined and metabolism-independent role of PEP in regulating resistance to antiangiogenic therapy by functioning as an endogenous HDAC1 inhibitor.

Keywords: Oncometabolism, Cancer therapeutic resistance, Colorectal cancer, Metabolomics

Immunoassay

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Figure Source:  Nat Metab. 2023 Sep 4. doi: 10.1038/s42255-023-00883-y.