[Cited FineTest Elisa Kit] Current Research on Neuroendocrinology and Metabolism

FineTest Elisa kit contributes to the research on neuroendocrinology and metabolism. The immunoassay is designed to measure Testosterone, LH, and FSH concentration in serum.

Publication Details
Article Title: Perfluorooctanesulfonic acid exposure altered hypothalamic metabolism and disturbed male fecundity
Journal Title: Science of The Total Environment
DOI: 10.1016/j.scitotenv.2022.156881
IF: 10.753
PMID: 35753445

Abstract: Previous studies have examined the effects of perfluorooctanesulfonic acid (PFOS) on disruption of the blood-testis barrier and spermatogenesis. Sertoli and Leydig cells were perturbed, resulting in a decrease in testosterone levels and sperm counts. However, the effects of PFOS on male fecundity are not limited to the testes. In this study, we demonstrated that oral PFOS exposure (1 μg/g BW and 5 μg/g BW) decreased the function of the Luteinizing hormone (LH)/Luteinizing hormone receptor (LHr) and decreased epididymal sperm motility. Consistently, testicular transcriptome analysis revealed that PFOS altered the expression of a cluster of genes associated with sperm motility and steroidogenesis. In mice exposed to PFOS, c-Fos immunostaining showed activation of the lateral septal nucleus (LS), paraventricular thalamus (PVT), locus coeruleus (LC), which are known to be related to anxiety-like behaviors. Metabolomic analyses of the hypothalamus revealed that exposure to PFOS perturbed the translation of proteins, as well as the biosynthesis of neurotransmitters and neuromodulators. Altogether, the activation of brain nuclei, shift of hypothalamic metabolome, and reduction of LH/LHr circuit resulted from PFOS exposure suggested the toxicant's systematic effects on male reproduction.

Keywords: PFOS, Locus coeruleus, Hypothalamus, Metabolome, Transcriptome, Metabolism

Immunoassay

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Figure Source: Sci Total Environ. 2022 Jun 23;844:156881. doi: 10.1016/j.scitotenv.2022.156881.

Fig.2.   PFOS exposure reduced sperm motility and serum LH level. (A) Computer-assisted sperm analysis (CASA) was used to analyze epididymal sperm quality; Serum testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were determined by ELISA. (B-C) PFOS did not affect the sperm count or the percentage of motile sperm. (D-F) PFOS at 1μg/g resulted in a decrease in the sperm motility reflected by a decreased VCL and VAP; PFOS at 5μg/g resulted in a decrease in the sperm motility reflected by a decreased VCL, VSL, and VAP; VCL, curvilinear velocity; VSL, straight-line velocity; VAP, average path velocity; data were presented as mean ± SEM, n = 7 for the control group, n = 6 for the 1μg/g PFOS group, n = 7 for the 5μg/g PFOS group; *P<0.05, **P<0.01,***P<0.001,ns, P>0.05 One-way ANOVA with Tukey's test. (G-I) No significant difference in serum testosterone levels among the three groups; PFOS at 5μg/g resulted in a decrease in the serum LH level; no significant difference in serum FSH among three groups; data were presented as mean ± SEM, n = 4 per group; *P<0.05, n, P>0.05 One-way ANOVA with Tukey's test.