FineTest ELISA kit contributes to the research on metabolic dysfunction. The immunoassay is designed to measure 3-Nitrotyrosine and protein carbonyl concentration in supernatant.
Publication Details
Article Title: Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis
Journal Title: Nature Communications
DOI: 10.1038/s41467-024-47553-5
IF: 16.6
PMID: 38615060
Abstract: Oxidative stress-induced lipid accumulation is mediated by lipid droplets (LDs) homeostasis, which sequester vulnerable unsaturated triglycerides into LDs to prevent further peroxidation. Here we identify the upregulation of lipopolysaccharide-binding protein (LBP) and its trafficking through LDs as a mechanism for modulating LD homeostasis in response to oxidative stress. Our results suggest that LBP induces lipid accumulation by controlling lipid-redox homeostasis through its lipid-capture activity, sorting unsaturated triglycerides into LDs. N-acetyl-L-cysteine treatment reduces LBP-mediated triglycerides accumulation by phospholipid/triglycerides competition and Peroxiredoxin 4, a redox state sensor of LBP that regulates the shuttle of LBP from LDs. Furthermore, chronic stress upregulates LBP expression, leading to insulin resistance and obesity. Our findings contribute to the understanding of the role of LBP in regulating LD homeostasis and against cellular peroxidative injury. These insights could inform the development of redox-based therapies for alleviating oxidative stress-induced metabolic dysfunction.
Keywords: Metabolic Dysfunction, Lipid Signalling, Metabolic Disorders, Oxidative Stress
Immunoassay
| FineTest Product | Sample | Species | Detection Target |
| 3-NT(3-Nitrotyrosine) ELISA Kit(EU2560) | supernatant | mouse | 3-NT |
| PC(Protein Carbonyl) ELISA Kit(EU2629) | PC |
Validated Image
Figure Source: Nat Commun. 2024 Apr 13;15(1):3213. doi: 10.1038/s41467-024-47553-5.