[Cited FineTest ELISA Kit] Current Research on Cardiomyopathy and Diabetes

FineTest ELISA kit contributes to the research on cardiomyopathy and diabetes. The immunoassay is designed to measure 4-HNE concentration in supernatant.

Publication Details
Article Title: Inhibition of fatty acid uptake by TGR5 prevents diabetic cardiomyopathy
Journal Title: Nature Metabolism
DOI: 10.1038/s42255-024-01036-5
IF: 20.8
PMID: 38698281

Abstract: Diabetic cardiomyopathy is characterized by myocardial lipid accumulation and cardiac dysfunction. Bile acid metabolism is known to play a crucial role in cardiovascular and metabolic diseases. Takeda G-protein-coupled receptor 5 (TGR5), a major bile acid receptor, has been implicated in metabolic regulation and myocardial protection. However, the precise involvement of the bile acid-TGR5 pathway in maintaining cardiometabolic homeostasis remains unclear. Here we show decreased plasma bile acid levels in both male and female participants with diabetic myocardial injury. Additionally, we observe increased myocardial lipid accumulation and cardiac dysfunction in cardiomyocyte-specific TGR5-deleted mice (both male and female) subjected to a high-fat diet and streptozotocin treatment or bred on the diabetic db/db genetic background. Further investigation reveals that TGR5 deletion enhances cardiac fatty acid uptake, resulting in lipid accumulation. Mechanistically, TGR5 deletion promotes localization of CD36 on the plasma membrane through the upregulation of CD36 palmitoylation mediated by the palmitoyl acyltransferase DHHC4. Our findings indicate that the TGR5-DHHC4 pathway regulates cardiac fatty acid uptake, which highlights the therapeutic potential of targeting TGR5 in the management of diabetic cardiomyopathy.

Keywords: Heart failure, Metabolic diseases, Cardiomyopathy and diabetes, Cardiovascular disease, Heart diseases

Immunoassay

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Figure Source: Nat Metab. 2024 May 2. doi: 10.1038/s42255-024-01036-5.