FineTest ELISA kit contributes to the research on bone resorption treatment. The immunoassay is designed to arachidonic acid and 12-HETE level in mice serum or cell supernatant.
Article Title: Pla2g7 regulates bone homeostasis via Alox12/12-HETE/Gpr31 signaling axis
Journal Title: Nature Communications
DOI: 10.1038/s41467-025-66285-8
IF: 15.7
PMID: 41372218
Abstract: Bone homeostasis mainly depends on the equilibrium of osteoclasts and osteoblasts, overactivated osteoclasts play a pivotal role in the progression of osteoporosis. Here, we revealed that Pla2g7 (phospholipase A2 group VII) was positively correlated with bone resorption in clinic. By single-cell RNA-seq data analysis, Pla2g7 was found highly enriched in osteoclasts along the developmental trajectory, which promoted osteoclast differentiation. Inhibition of Pla2g7 by Darapladib impaired both human and mice osteoclast differentiation, meanwhile, Pla2g7-deficient mice showed higher bone mass and restored the ovariectomy-induced bone loss. Mechanistically, we identified that Alox12 (arachidonate 12-lipoxygenase) mediated-arachidonic acid metabolism is a key determinant in Pla2g7 enhanced osteoclast differentiation. Its metabolite 12-HETE (12-hydroxyeicosatetraenoic acid) activated Gpr31 to regulate osteoclast formation via p38 MAPK pathway and mitochondrial energy metabolism. Collectively, our study uncovers an Alox12/12-HETE/Gpr31 axis that regulates Pla2g7-induced osteoclast differentiation, and provides a new insight for osteoporosis treatment.
Keywords: Central control of bone remodelling, Drug regulation, Osteoporosis, Bone resorption
Immunoassay
| FineTest Product | Sample | Species | Detection Target |
| Mouse AA(Arachidonic Acid) ELISA Kit(EM2143) | serum | Mouse | AA |
| 12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit(EU3131) | cell supernatant | 12-HETE |
Validated Image
Figure Source: Nat Commun. 2025 Dec 10;16(1):11449. doi: 10.1038/s41467-025-66285-8.