FineTest ELISA kit contributes to the research on biomaterials for stem cell therapy. The immunoassay is designed to measure SCF, TPO and Flt3L concentration respectively.
Publication Details
Article Title: In vitro expansion of hematopoietic stem cells in a porous hydrogel-based 3D culture system
Journal Title: Acta Biomaterialia
DOI: 10.1016/j.actbio.2023.01.057
IF: 10.633
PMID: 36754271
Abstract: Hematopoietic stem cell (HSC) transplantation remains the most effective therapy for hematologic and lymphoid disorders. However, as the primary therapeutic cells, the source of HSCs has been limited due to the scarcity of matched donors and difficulties in ex vivo expansion. Here, we described a facile method to attempt the expansion of HSCs in vitro through a porous alginate hydrogel-based 3D culture system. We used gelatin powders as the porogen to create submillimeter-scaled pores in alginate gel bulk while pre-embedding naïve HSCs in the gel phase. The results indicated that this porous hydrogel system performed significantly better than those cultured via conventional suspension or encapsulation in non-porous alginate hydrogels in maintaining the phenotype and renewability of HSCs. Only the porous hydrogel system achieved a two-fold growth of CD34+ cells within seven days of culture, while the number of CD34+ cells in the suspension system and nonporous hydrogel showed different degrees of attenuation. The expansion efficiency of the porous hydrogel for CD34+CD38- cells was more than 2.2 times that of the other two systems. Mechanistic study via biophysical analysis revealed that the porous alginate system was competent to reduce the electron capture caused by biomaterials, decrease cellular oxygen stress, avoid oxidative protection, thus maintaining the cellular phenotype of the CD34+ cells. The transcriptomic analysis further suggested that the porous alginate system also upregulated the TNF signaling pathway and activated the NF-κB signaling pathway to promote the CD34+ cells' survival and maintain cellular homeostasis so that renewability was substantially favoured.
Keywords: 3D culture; Hematopoietic stem cell; alginate; in vitro expansion; porous hydrogel
Immunoassay
| FineTest Product | Species | Detection Target |
| Human KitLG(Kit ligand) ELISA Kit(EH0514) | Human | SCF |
| Human TPO(Thrombopoietin) ELISA Kit(EH0422) | TPO | |
| Human Flt3L(FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit(EH3074) | Flt3L |
Illustration
Figure Source: Acta Biomater. 2023 Feb 6;S1742-7061(23)00067-3. doi: 10.1016/j.actbio.2023.01.057.