FineTest ELISA kit contributes to the research on autophagy and alzheimer's disease. The immunoassay is designed to measure BNIP3L concentration in CSF and serum.
Publication Details
Article Title: Alterations of human CSF and serum-based mitophagy biomarkers in the continuum of Alzheimer disease
Journal Title: Autophagy
DOI: 10.1080/15548627.2024.2340408
IF: 13.3
PMID: 38695174
Abstract: Defective mitophagy is consistently found in postmortem brain and iPSC-derived neurons from Alzheimer disease (AD) patients. However, there is a lack of extensive examination of mitophagy status in serum or cerebrospinal fluid (CSF), and the clinical potential of mitophagy biomarkers has not been tested. We quantified biomarkers of mitophagy/autophagy and lysosomal degradation (PINK1, BNIP3L and TFEB) in CSF and serum from 246 individuals, covering mild cognitive impairment due to AD (MCI-AD, n = 100), dementia due to AD (AD-dementia, n = 100), and cognitively unimpaired individuals (CU, n = 46), recruited from the Czech Brain Aging Study. Cognitive function and brain atrophy were also assessed. Our data show that serum and CSF PINK1 and serum BNIP3L were higher, and serum TFEB was lower in individuals with AD than in corresponding CU individuals. Additionally, the magnitude of mitophagy impairment correlated with the severity of clinical indicators in AD patients. Specifically, levels of PINK1 positively correlated with phosphorylated (p)-MAPT/tau (181), total (t)-MAPT/tau, NEFL (neurofilament light chain), and NRGN (neurogranin) levels in CSF and negatively with memory, executive function, and language domain. Serum TFEB levels negatively correlated with NEFL and positively with executive function and language. This study reveals mitophagy impairment reflected in biofluid biomarkers of individuals with AD and associated with more advanced AD pathology.
Keywords: Autophagy and Alzheimer's Disease, BNIP3L, mild cognitive impairment, mitophagy, PINK1, TFEB
Immunoassay
| FineTest Product | Sample | Species | Detection Target |
| Human BNIP3L(BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like) ELISA Kit(EH6731) | CSF and serum | human | BNIP3L |
Validated Image
Figure Source: Autophagy. 2024 May 2:1-11. doi: 10.1080/15548627.2024.2340408.