FineTest ELISA kit contributes to the research on atherosclerosis and stroke. The immunoassay is designed to measure DLL and Jag level in plasma.
Article Title: Brain ischemia causes systemic Notch1 activity in endothelial cells to drive atherosclerosis
Journal Title: Immunity
DOI: 10.1016/j.immuni.2024.07.002
IF: 25.5
PMID: 39079536
Abstract: Stroke leads to persistently high risk for recurrent vascular events caused by systemic atheroprogression that is driven by endothelial cell (EC) activation. However, whether and how stroke induces sustained pro-inflammatory and proatherogenic endothelial alterations in systemic vessels remain poorly understood. We showed that brain ischemia induces persistent activation, the upregulation of adhesion molecule VCAM1, and increased senescence in peripheral ECs until 4 weeks after stroke onset. This aberrant EC activity resulted from sustained Notch1 signaling, which was triggered by increased circulating Notch1 ligands DLL1 and Jagged1 after stroke in mice and humans. Consequently, this led to increased myeloid cell adhesion and atheroprogression by generating a senescent, pro-inflammatory endothelium. Notch1- or VCAM1-blocking antibodies and the genetic ablation of endothelial Notch1 reduced atheroprogression after stroke. Our findings revealed a systemic machinery that induces the persistent activation of peripheral ECs after stroke, which paves the way for therapeutic interventions or the prevention of recurrent vascular events following stroke.
Keywords: atherosclerosis and stroke, brain ischemia, endothelial cells, inflammation
Immunoassay
| FineTest Product | Sample | Species | Detection Target |
| Mouse Dll1(Delta-like protein 1)ELISA Kit(EM6642) | plasma | Mouse | Dll1 |
| Mouse Dll3(Delta-like protein 3)ELISA Kit(EM2010) | Dll3 | ||
| Mouse Dll4(Delta-like protein 4)ELISA Kit(EM2011) | Dll4 | ||
| Mouse Jag1(Protein jagged-1)ELISA Kit(EM1918) | Jag1 | ||
| Mouse Jag2(Protein jagged-2)ELISA Kit(EM7320) | Jag2 | ||
| Mouse Hmgb1(High mobility group protein B1) ELISA Kit(EM0382) | Hmgb1 | ||
| Human JAG1(Protein jagged-1)ELISA Kit(EH4500) | Human | Jag1 | |
| Human DLL1(Delta-like protein 1) ELISA Kit(EH0115) | Dll1 |
Validated Image
Figure Source: Immunity. 2024 Jul 23:S1074-7613(24)00351-0. doi: 10.1016/j.immuni.2024.07.002.