High IF Citations

【Cited FineTest Protein】 Current Research on Stem Cell Therapy for Cardiovascular Disease

FineTest recombinant protein contributes to the research on stem cell therapy. Tb4-his-tag protein is used to determine the duration of the Tb4 release in vivo.

Publication Details
Article Title: Thymosin β4 Increases Cardiac Cell Proliferation, Cell Engraftment, and the Reparative Potency of Human Induced-pluripotent Stem Cell-derived Cardiomyocytes in a Porcine Model of Acute Myocardial Infarction
Journal Title: Theranostics
DOI: 10.7150/thno.56757
IF: 11.556
PMID: 34335970

Abstract:      Previous studies have shown that human embryonic stem cell-derived cardiomyocytes improved myocardial recovery when administered to infarcted pig and non-human primate hearts. However, the engraftment of intramyocardially delivered cells is poor and the effectiveness of clinically relevant doses of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in large animal models of myocardial injury remains unknown. Here, we determined whether thymosin β4 (Tb4) could improve the engraftment and reparative potency of transplanted hiPSC-CMs in a porcine model of myocardial infarction (MI).
Keywords:   Myocardial Infarction, Stem Cell Therapy, Microsphere

Immunoassay

FineTest Product Sample Detection Target Species
Recombinant Human Thymosin beta 4 (P5500) serum and heart tissue lysate Thymosin beta 4 Human

The concentration of his-tag in serum and heart tissue lysate were determined using a His Tag ELISA Detection Kit.

Validated Image

stem cell therapy

Figure Source: Theranostics, 2021 Jun 26;11(16):7879-7895. doi: 10.7150/thno.56757.

Figure 1. Tb4 protects hiPSC-CM against hypoxic injury in vitro. To determine cyto-protective effect of Tb4, 2x105 hiPSC-CMs/well were cultured in 12-well plate. After washing thrice with DPBS, hiPSC-CMs were cultured in 500 μL Hanks balanced salt solution (HBSS) supplemented with or without Tb4 protein and cultured in an incubator with hypoxia condition for 24 h: 5% CO2, 94% N2, and 1% O2. (A) The concentrations of lactate dehydrogenase (LDH) in the cell culture medium were measured in the absence or presence of Tb4 and presented as a percentage of the measurements obtained in the absence of Tb4 (n = 6 for each concentration. One-way ANOVA analysis). (B) The concentration of DNA fragments in the cell culture medium were measured in the absence or presence of 600 ng/mL Tb4 (n = 6 for each sample. Independent T-test). (C) hiPSC-CMs cultured in HBSS medium supplemented without or with 600 ng/mL Tb4 were TUNEL-stained and counter-stained with DAPI after 24 hours’ hypoxia. (α-SA: α-sarcomere actin. Bar = 100 μm). (D) hiPSC-CM apoptosis was quantified as the number of TUNEL+hiPSC-CMs over the total hiPSC-CMs per field (n = 3 for each sample. Independent T-test). (E) Representative Western Blot images of hiPSC-CMs cultured in HBSS medium supplemented without or with 600 ng/mL Tb4 for protein expressions of pAKT, AKT, and Bcl-XL. Protein expression level of GAPDH was used as an internal control. Cells were cultured under hypoxic condition and were harvested at 0, 1, 3, and 6 h after treatment. (F) Quantification of pAKT protein expressions, which were expressed as percentages of AKT protein levels after normalized with GAPDH protein. (n = 5 for each sample. Independent T-test). (G) Quantification of Bcl-XL protein expressions, which were presented as percentages of GAPDH protein levels. (n = 5 for each sample. Independent T-test). (*: p < 0.05; **: p < 0.01; ***: p < 0.001). Values are presented as the means ± SD.