FineTest Elisa kit contributes to the research on SARS-CoV-2. The immunoassay is designed to measure Anti-SARS-CoV-2 IgG concentration in ferret serum.
Publication Details
Article Title: Age-dependent Pathogenic Characteristics of SARS-CoV-2 Infection in Ferrets
Journal Title: Nature Communications
DOI: 10.1038/s41467-021-27717-3
IF: 15.805
PMID: 35013229
Abstract: While the seroprevalence of SARS-CoV-2 in healthy people does not differ significantly among age groups, those aged 65 years or older exhibit strikingly higher COVID-19 mortality compared to younger individuals. To further understand differing COVID-19 manifestations in patients of different ages, three age groups of ferrets are infected with SARS-CoV-2. Although SARS-CoV-2 is isolated from all ferrets regardless of age, aged ferrets (≥3 years old) show higher viral loads, longer nasal virus shedding, and more severe lung inflammatory cell infiltration, and clinical symptoms compared to juvenile (≤6 months) and young adult (1-2 years) groups. Furthermore, direct contact ferrets co-housed with the virus-infected aged group shed more virus than direct-contact ferrets co-housed with virus-infected juvenile or young adult ferrets. Transcriptome analysis of aged ferret lungs reveals strong enrichment of gene sets related to type I interferon, activated T cells, and M1 macrophage responses, mimicking the gene expression profile of severe COVID-19 patients. Thus, SARS-CoV-2-infected aged ferrets highly recapitulate COVID-19 patients with severe symptoms and are useful for understanding age-associated infection, transmission, and pathogenesis of SARS-CoV-2.
Immunoassay
| FineTest Product | Sample | Detection Target | Species |
| Human anti-SARS-CoV2(N) IgG ELISA Kit (EH4397) | Serum | Anti-SARS-CoV-2 IgG | Human |
Validated Image
Figure Source: Nat Commun, 2022 Jan 10;13(1):21. doi: 10.1038/s41467-021-27717-3.
Fig. 4. Serum neutralizing antibody (NAb) and IgG antibody titers in different ages of ferrets. The NAb titers against SARS-CoV-2 NMC-nCoV02 (100 TCID50) among different age groups was measured in Vero cells with serially diluted ferret sera collected at 0, 12, and 21 dpi (a). The IgG titers among different age groups in serially diluted sera collected at 0, 12, and 21 dpi (b). Groups with the same letter are of the same subgroup in the post-hoc analysis. Lower case letters indicate significant differences at each time point (P values are a vs b: 0.00017 or 0.00168 (a), a vs b: 0.03 or 0.0044 (b)), while upper case letters indicate significant differences of area under the curve as change over the entire period (P values are A vs B: 0.0012 or 0.0233 (a), A vs B: 0.03 (b)). The area under the curve was calculated using Kruskal–Wallis with Bonferroni as a post-hoc analysis. Data were analyzed in GraphPad Prism 9.1.2. Data are presented as mean ± SEM (n = 3). The limit of detection for NAb titers and OD450 nm value is 0.5, indicated by the dotted line. Source data are provided as a Source Data file.