【Cited FineTest Elisa Kit】 Current Research on Cell Biology and Intestinal Stem Cells

FineTest Elisa kit contributes to the research on cell biology and intestinal stem cells. The immunoassay is designed to measure PGE2 concentration in culture supernatant.

Publication Details
Article Title: Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons
Journal Title: Cell Research
DOI: 10.1038/s41422-022-00645-7
IF: 25.924
PMID: 35379903

Abstract:  Lgr5⁺ intestinal stem cells (ISCs) reside within specialized niches at the crypt base and harbor self-renewal and differentiation capacities. ISCs in the crypt base are sustained by their surrounding niche for precise modulation of self-renewal and differentiation. However, how intestinal cells in the crypt niche and microbiota in enteric cavity coordinately regulate ISC stemness remains unclear. Here, we show that ISCs are regulated by microbiota and niche enteric serotonergic neurons. The gut microbiota metabolite valeric acid promotes Tph2 expression in enteric serotonergic neurons via blocking the recruitment of the NuRD complex onto Tph2 promoter. 5-hydroxytryptamine (5-HT) in turn activates PGE2 production in a PGE2⁺ macrophage subset through its receptors HTR2A/3 A; and PGE2 via binding its receptors EP1/EP4, promotes Wnt/β-catenin signaling in ISCs to promote their self-renewal. Our findings illustrate a complex crosstalk among microbiota, intestinal nerve cells, intestinal immune cells and ISCs, revealing a new layer of ISC regulation by niche cells and microbiota.

Keywords: Cell Self Renewal, Gastrointestinal Microbiome, Intestinal Mucosa, Macrophages, Serotonergic Neurons, Stem Cells

Immunoassay

Validated Image

Figure Source: Cell Res, 2022 Apr 4. doi: 10.1038/s41422-022-00645-7.

Figure 7.  Characterization of PGE2⁺ macrophage.: l. ELISA detection for PGE2 production in indicated treated macrophages. n=4 independent experiments were performed.