Products
beta actin antibody
| Synonyms： | ACTB antibody, Actin antibody, actin antibody, beta antibody, Actin antibody, cytoplasmic 1 antibody, B actin antibody, Beta actin antibody, F actin antibody, β actin antibody | ||
| Catalogue No.： | FNab00869 | Reactivity： | Human, Mouse, Rat |
| Host： | Rabbit | Tested Application： | ELISA, IF, WB, IHC, IP |
| Clonality： | polyclonal | Isotype： | IgG |
- SPECIFICATIONS
- CITATIONS
- Product Name
- beta actin antibody
- Catalogue No.
- FNab00869
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- actin, beta
- Alternative Names
- ACTB antibody, Actin antibody, actin antibody, beta antibody, Actin antibody, cytoplasmic 1 antibody, B actin antibody, Beta actin antibody, F actin antibody, β actin antibody
- UniProt ID
- P60709
- Observed MW
- 42 kDa
Application
- Tested Applications
- ELISA, IF, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:5000; IHC: 1:20-1:500; IF: 1:50-1:500;IP: 1:500-1:2000
Validated Images
Various lysates were subjected to SDS PAGE followed by western blot with FNab00869(beta actin Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human heart using FNab00869(ACTB antibody) at dilution of 1:100
- Background
- Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
IF: 14
- Journal:
- Biomaterials
- Author:
- Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing, 400044, PR China
- Cited Date:
- 2023-09-15
- Product:
IF: 5.396
- Journal:
- Food & Function
- Cited Date:
- 2021-08-27
- Product:
IF: 4.847
- Journal:
- Scientific Reports
- Cited Date:
- 2020-06-23
- Product:
IF: 4.009
- Journal:
- Frontiers in Bioscience-Landmark
- Cited Date:
- 2022-05-06
- Product:
IF: 3.701
- Journal:
- Journal of Functional Foods
- Cited Date:
- 2021-02-06
- Product:
IF: 3.267
- Journal:
- Molecules
- Cited Date:
- 2020-09-11
- Product:
IF: 2.976
- Journal:
- Current Issues in Molecular Biology
- Cited Date:
- 2022-10-21
- Product:
IF: 2.482
- Journal:
- Biological Research
- Cited Date:
- 2020-05-04
- Product:
- Journal:
- Toxicology Reports
- Author:
- Faculty of Applied Sciences, State University of Campinas, Limeira, SP, Brazil
- Cited Date:
- 2023-06-23
- Product:
- Journal:
- Research Square
- Author:
- North Sichuan Medical College
- Cited Date:
- 2023-09-01
- Product:
