anti- beta actin antibody

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Product Information
Product name

anti- beta actin antibody

Alternative name

beta actin Antibody

Catalogue No.

FNab00869

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Description

beta actin antibody: Rabbit Polyclonal antibody to beta actin

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.

Immunogen Information
Immunogen

actin, beta

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin

Calculated MW

42 kDa

Application
Specificity

Human, Mouse, Rat

Tested Application

ELISA, IF, WB, IHC

Recommended dilution

WB: 1:500-1:2000; IHC: 1:20-1:500; IF: 1:10-1:100

Uniprot information
Gene Name

beta actin

Uniprot ID

P60709

Images

anti- beta actin antibody 1

Immunohistochemistry of paraffin-embedded human heart using FNab00869(ACTB antibody) at dilution of 1:100


anti- beta actin antibody 1

C6 cells were subjected to SDS PAGE followed by western blot with FNab00869(beta actin Antibody) at dilution of 1:2000

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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Hotline

027-87384275
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