Loading Control Antibodies

Loading control antibodies are widely used in WB to detect loading control protein expression level and ensure accuracy of experimental results by correcting the error during protein quantitation and sample loading. Besides, loading control can be used as blank control to detect protein transmembrane situation, the staining or ECL process of western blot.

FineTest provides a complete set of polyclonal and monoclonal loading control antibodies with high specificity and wide applications, which can be used in most qualitative, quantitative and localization analysis of proteins. We provide common and rare species loading control antibodies(e.g .human, mouse, rat etc) as well as custom services.

View All Loading Control Antibodies

FNab03342 GAPDH antibody:

FNab03342

FNab00880 Beta Tubulin antibody:

FNab00880.

How to Choose Loading Control Antibodies?

Molecular Weight

 

Expression Position

For an obvious band effect, loading control protein needs to be at least 5 KD different from target protein.

Loading control proteins have different expression levels according to different cell organs. It also needs to consider sample type while choosing suitable loading control antibodies.

Sample Type

Loading Control

MW(kDa)

cell membrane

ATP1A1

100-110

mitochondrion

VDAC1

31

COXIV

17-20

endoplasmic reticulum

calreticulin

55-60

Golgi apparatus

GM130

130

 

**Loading control proteins MW and expression level in different cell organs.

Sample Type

Loading Control

MW(kDa)

lysosomes

LAMP2

100-120

blood

Transferrin

77

whole-cell, cytoplasm

β-actin, α-actin

42

GAPDH

36

β-tubulin, α-tubulin

50-55

Vinculin

(for high MW)

117

nucleus

Lamin B1

66

TBP

38

YY1

65-70

Histone H3

18

PCNA

38

HDAC1

55

 

Brain

Spleen

Skeletal Muscle

Kidney

Pancreas

Testis

Skin

Eye

Heart

Lung

α-Tubulin

Low

β-Actin

Low

Low

β-Tubulin

Low

Low

COX IV

GAPDH

Histone H3

Low

Low

β3-Tubulin

Low

Low

Low

Low

**Loading control protein expression level in different mouse tissues.

Advantages of FineTest Antibodies

A Complete Set Of

Loading Control Antibodies

(MW: 15kDa - 130kDa)

 

Strict QC,

Various Validations,

High Performance

 

Flexible Size

Recommended Loading Control Antibodies

Catalogue No.

Product Name

MW(kDa)

Antibody Type

Applications

FNab00869

anti- beta actin antibody

42

Rabbit pAb

ELISA, IF, WB, IHC

FNab00873

anti- beta actin antibody

42

Mouse mAb

ELISA, WB, IHC, IF

FNab03342

anti- GAPDH antibody

36

Rabbit pAb

ELISA, IHC, WB

FNab03343

anti- GAPDH antibody

36

Mouse mAb

ELISA, IHC, WB, IF, FC, IP

FNab00880

anti- Beta Tubulin antibody

50-55

Rabbit pAb

ELISA, WB, IF, IP, IHC, FC

FNab09105

anti- Tubulin-beta antibody

50-55

Mouse mAb

ELISA, WB, IHC, IF, IP

Citations