Loading Control Antibodies - FineTest ELISA Kit | FineTest Antibody

Loading control antibodies are widely used in WB to detect loading control protein expression level and ensure accuracy of experimental results by correcting the error during protein quantitation and sample loading. Besides, loading control can be used as blank control to detect protein transmembrane situation, the staining or ECL process of western blot.

FineTest provides a complete set of polyclonal and monoclonal loading control antibodies with high specificity and wide applications, which can be used in most qualitative, quantitative and localization analysis of proteins. We provide common and rare species loading control antibodies(e.g .human, mouse, rat etc) as well as custom services.

View All Loading Control Antibodies

Human kidney tissue, hela cell lysate

anti- GAPDH antibody
Cat NO.: FNab03343
Reactivity: Human, Mouse, Rat
Application: ELISA, IHC, WB, IF, FC, IP

Human testis tissue, hela cell lysate

loading control antibodies - alpha Tubulin

anti- alpha Tubulin antibody
Cat NO.: FNab00334
Reactivity: Human, Mouse, Rat
Application: ELISA, IHC, IF, WB

Human pancreatic cancer tissue, HEK-293 cell lysate

anti- VDAC1/Porin antibody
Cat NO.: FNab09385
Reactivity: Human, Mouse
Application: ELISA, WB, IHC, IF

How to Choose Loading Control Antibodies?

Molecular Weight

Expression Position

For an obvious band effect, loading control protein needs to be at least 5 KD different from target protein.

Loading control proteins have different expression levels according to different cell organs. It also needs to consider sample type while choosing suitable loading control antibodies.

Sample Type	Loading Control	MW(kDa)
cell membrane	ATP1A1	100-110
mitochondrion	VDAC1	31
mitochondrion	COXIV	17-20
endoplasmic reticulum	calreticulin	55-60
Golgi apparatus	GM130	130

**Loading control proteins’ MW and expression level in different cell organs.

Sample Type	Loading Control	MW(kDa)
lysosomes	LAMP2	100-120
blood	Transferrin	77
whole-cell, cytoplasm	β-actin, α-actin	42
	GAPDH	36
	β-tubulin, α-tubulin	50-55
	Vinculin (for high MW)	117
nucleus	Lamin B1	66
	TBP	38
	YY1	65-70
	Histone H3	18
	PCNA	38
	HDAC1	55

	Brain	Spleen	Skeletal Muscle	Kidney	Pancreas	Testis	Skin	Eye	Heart	Lung
α-Tubulin	√	√	√	√	Low	√	√	√	√	√
β-Actin	√	√	Low	√	√	√	√	√	Low	√
β-Tubulin	√	√	Low	√	Low	√	√	√	√	√
COX IV	√	√	√	√	√	√	√	√	√	√
GAPDH	√	√	√	√	√	√	√	√	√	√
Histone H3	√	√	√	√	√	√	Low	Low	√	√
β3-Tubulin	√	Low	√	Low	√	√	Low	√	Low	√

**Loading control protein expression level in different mouse tissues.

Advantages of FineTest Antibodies

A Complete Set Of

Loading Control Antibodies

(MW: 15kDa - 130kDa)

Strict QC,

Various Validations,

High Performance

Flexible Size

Recommended Loading Control Antibodies

Catalogue No.	Product Name	MW(kDa)	Antibody Type	Applications
FNab00869	anti- beta actin antibody	42	Rabbit pAb	ELISA, IF, WB, IHC
FNab00873	anti- beta actin antibody	42	Mouse mAb	ELISA, WB, IHC, IF
FNab03342	anti- GAPDH antibody	36	Rabbit pAb	ELISA, IHC, WB
FNab03343	anti- GAPDH antibody	36	Mouse mAb	ELISA, IHC, WB, IF, FC, IP
FNab00880	anti- Beta Tubulin antibody	50-55	Rabbit pAb	ELISA, WB, IF, IP, IHC, FC
FNab09105	anti- Tubulin-beta antibody	50-55	Mouse mAb	ELISA, WB, IHC, IF, IP

Citations

Article Title: Anti-hyperuricemic effects of three theaflavins isolated from black tea in hyperuricemic mice

IF: 3.197

Journal Title: Journal of Functional Foods

Target: GAPDH, URAT1, OAT1, GLUT9
Article Title: lncRNA NEAT1 Facilitates Cell Proliferation, Invasion and Migration by Regulating CBX7 and RTCB in Breast Cancer

IF: 3.046

Journal Title: OncoTargets and Therapy

Target: GAPDH, RtcB
Article Title: miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2

IF: 2.482

Journal Title: Biological Research

Target: beta actin
Article Title: Inhibition of NLRP3 and Golph3 ameliorates diabetes-induced neuroinflammation in vitro and in vivo

IF: 5.955

Journal Title: Aging

Target: GOLGA2/GM130, GOLPH3, Vimentin