Rat IL-10(Interleukin-10) ELISA Kit

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  • Specifications
Product name

Rat IL-10(Interleukin-10) ELISA Kit

Catalogue No.

ER0033

Detection method

Sandwich ELISA, Double Antibody

Application

IL-10 ELISA Kit allows for the in vitro quantitative determination of IL-10 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Rat IL-10(Interleukin-10) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-10 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-10 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-100 95
EDTA plasma(n=5) 91-103 96
heparin plasma(n=5) 86-101 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-98% 87-104% 85-100% 85-99%
EDTA plasma(n=5) 83-93% 82-92% 82-97% 82-101%
heparin plasma(n=5) 83-97% 82-91% 80-92% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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